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Table 1 List of primers used for bacterial identification and detection of MSCRAMMs genes (fnbA, fnbB and cna), biofim control genes (icaA and icaD) and exoenzymes genes (sspA, sspB and geh)

From: Comparative study of virulence factors among methicillin resistant Staphylococcus aureus clinical isolates

Target gene Sequence (5’-3’) aT (°C) Amplified fragment Amplicon size (bp) Reference
Sa442 5’-AATCTTTGTCGGTACACGATATTCTTCACG-3’ 5’-CGTAATGAGATTTCAGTAAATACAACA-3’ 55 Sa442 protein 108 [7]
mecA 5’-ACCAGATTACAACTTCACCAGG-3’ 5’-CCACTTCATATCTTGTAACG-3’ 45 Penicillin binding protein 2a 162 [7]
icaA 5’-ACACTTGCTGGCGCAGTCAA-3’ 5’-TCTGGAACCAACATCCAACA-3’ 55 Intercellular adhesion A 188 [24]
icaD 5’-ATGGTCAAGCCCAGACAGAG-3’ 5’-AGTATTTTCAATGTTTAAAGCAA-3’ 55 Intercellular adhesion D 198 [24]
Can 5’-GTCAAGCAGTTATTAACACCAGAC-3’ 5’-AATCAGTAATTGCACTTTGTCCACTG-3’ 62 Collagen adhesion 192 [36]
fnbA 5’-CATAAATTGGGAGCAGCATCA-3’ 5’-ATCAGCAGCTGAATTCCCATT-3’ 62 Fibronectin binding protein A 191 [36]
fnbB 5’-GTAACAGCTAATGGTCGAATTGATACT-3’ 5’-CAAGTTCGATAGGAGTACTATGTTC-3’ 62 Fibronectin binding protein B 201 [36]
sspA 5’-GACAACAGCGACACTTGT 3’ 3’-AGTATCTTTACCTACAACTACA-5’ 45 Serine protease 292 [40]
sspB 5’-TGAAGAAGATGGCAAAGTTAG-3’ 3’-TTGAGATACACTTTGTGCAAG-5’ 47 Cysteine protease 493 [40]
Geh 5’-GCACAAGCCTCGG -3’ 3’-GACGGGGGTGTAG-5’ 40 Lipase 473 [45]
  1. aT annealing temperature