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Table 4 Comparison of 9-plex PCR with CBS in-house MALDI-TOF database and sequencing of D1/D2 LSU rDNA

From: Identification of nine cryptic species of Candida albicans, C. glabrata, and C. parapsilosis complexes using one-step multiplex PCR

Species Multiplex PCR MALDI-TOF Sequencing
C. albicans (n = 128) C. albicans (n = 128) C. albicans (n = 128) C. albicans (n = 128)
C. dubliniensis (n = 4) C. dubliniensis (n = 4) C. dubliniensis (n = 4) C. dubliniensis (n = 4)
C. africana (n = 3) C. africana (n = 3) C. albicans (n = 3) C. africana (n = 3)a
C. glabrata (n = 87) C. glabrata (n = 87) C. glabrata (n = 87) C. glabrata (n = 87)
C. nivariensis (n = 2) C. nivariensis (n = 2) C. nivariensis (n = 2) C. nivariensis (n = 2)
C. bracarensis (n = 2) C. bracarensis (n = 2) C. bracarensis (n = 2) C. bracarensis (n = 2)
C. parapsilosis (n = 28) C. parapsilosis (n = 28) C. parapsilosis (n = 28) C. parapsilosis (n = 28)
C. orthopsilosis (n = 6) C. orthopsilosis (n = 6) C. orthopsilosis (n = 6) C. orthopsilosis (n = 6)
C. metapsilosis (n = 20) C. metapsilosis (n = 20) C. metapsilosis (n = 20) C. metapsilosis (n = 20)
  1. a Similarity between C. albicans and C. africana using sequencing of D1/D2 LSU rDNAwas more than 99%
  2. This library has been enriched with a diverse range of yeast species utilized in brewery