You are viewing the site in preview mode

Skip to main content

Advertisement

Table 1 Cytoadherence and apoptosis of HLEC and hCMEC/D3 mediated by P. falciparum infected red blood cell

From: Comparison of apoptosis in human primary pulmonary endothelial cells and a brain microvascular endothelial cell line co-cultured with Plasmodium falciparum field isolates

Pf isolate Clinical status Number of pRBC adhered per 700 cells Mean OD direct contact mediated apoptosis (SD) Mean OD soluble factor mediated apoptosis (SD)
hCMEC/D3 HLEC HLEC hCMEC/D3 HLEC hCMEC/ D3
F1 UN 1 641 0.99 (0.05) 9.08 (0.05) 2.23 (0.09) 8.04 (0.61)
F2 UN 1 1 1.57 (0.12) 8.73 (0.62) 0.83 (0.04) 5.41 (0.07)
F3 UN 25 41 0.86 (0.04) 1.12 (0.09) 0.71 (0/09) 0.46 (0.05)
3D71 - 254 415 3.15 3.79 1.25 4.52
RBC1 - 1 0 0.39 0.49 0.25 0.41
F4 UN 3152 201 2.79 (0.07) 1.68 (0.14) 0.74 (0.04) 0.93 (0.05)
F5 UN 806 54 1.79 (0.08) 1.57 (0.11) 0.62 (0.02) 0.63 (0.18)
3D72 - 279 361 3.65 3.21 1.05 4.02
RBC2 - 3 8 0.67 0.23 0.42 0.29
F15 UN 69 81 6.39 (0.46) 11.37 (0.21) 2.23 (0.07) 8.59 (0.23)
F16 UN 3 9 1.00 (0.04) 0.68 (0.09) 0.41 (0.05) 1.70 (0.11)
F17 UN 9 41 0.82 (0.09) 0.84 (0.04) 0.73 (0.04) 8.73 (0.69)
3D73 - 291 541 3.19 3.52 1.88 4.45
RBC3 - 0 0 0.95 0.32 0.89 0.48
F19 UN 7 11 0.71 (0.03) 0.69 (0.06) 0.60 (0.05) 9.08 (0.09)
3D74 - 213 245 3.43 3.91 1.25 4.58
RBC4 - 10 6 0.20 0.88 0.11 0.61
F20 UN 51 157 2.55 (0.08) 0.92 (0.13) 0.63 (0.04) 0.54 (0.06)
F21 UN 0 1 2.07 (0.09) 0.95 (0.06) 1.56 (0.30) 0.59 (0.08)
F23 UN 5 54 2.87 (0.09) 0.89 (0.01) 1.09 (0.06) 0.74 (0.03)
3D75 - 259 354 3.00 3.25 1.56 4.12
RBC5 - 2 2 0.38 0.14 0.46 0.23
F25a SM 5040 2340 0.40 (0.12) 0.74 (0.04) 1.20 (0.10) 0.55 (0.04)
F26b SM 1 3 2.08 (0.19) 3.09 (0.06) 3.48 (0.03) 1.88 (0.15)
F27 UN 1652 224 0.63 (0.04) 0.32 (0.11) 1.09 (0.11) 0.66 (0.04)
F28 UN 3 5 2.07 (0.09) 0.42 (0.04) 3.53 (0.08) 0.88 (0.16)
3D76 - 368 463 3.65 4.01 1.12 4.85
RBC6 - 3 2 0.52 0.26 0.44 0.31
F29 UN 231 87 2.07 (0.10) 1.62 (0.06) 3.40 (0.10) 3.81 (0.11)
F30 UN 473 286 4.12 (0.14) 5.43 (0.26) 1.97 (0.04) 8.68 (0.13)
F31 UN 337 307 0.38 (0.12) 1.30 (0.34) 4.87 (0.10) 6.75 (0.18)
F33 UN 7 31 1.46 (0.09) 4.74 (0.20) 2.49 (0.06) 1.74 (0.05)
3D77 - 363 435 3.02 3.85 1.00 5.24
RBC7 - 0 1 0.54 0.45 0.35 0.15
F34 UN 10 67 1.10 (0.07) 2.23 (0.14) 1.84 (0.08) 2.73 (0.05)
F32 UN 70 157 1.05 (0.07) 4.32 (0.17) 1.07 (0.05) 1.91 (0.11)
3D78 - 440 522 3.75 4.12 1.89 5.87
RBC8 - 1 1 0.23 0.46 0.23 0.31
F35 UN 180 101 1.78 (0.11) 1.44 (0.10) 1.76 (0.14) 4.47 (0.09)
F36 UN 7 11 0.94 (0.07) 1.67 (0.08) 0.99 (0.01) 5.92 (0.09)
F37 UN 3 2 3.39 (0.30) 3.00 (0.15) 2.23 (0.04) 6.72 (0.10)
3D79 - 304 354 3.15 3.82 1.46 4.54
RBC9 - 3 0 0.65 0.24 0.69 0.36
F39 UN 1 21 1.57 (0.12) 1.53 (0.07) 0.44 (0.06) 1.03 (0.05)
F40 UN 70 196 3.79 (0.14) 3.50 (0.05) 0.50 (0.04) 0.99 (0.01)
3D710 - 343 398 3.05 3.81 0.96 4.21
RBC10 - - - 0.52 0.26 0.66 0.17
  1. Pf P. falciparum, UN uncomplicated malaria, SM severe malaria. The number of adherent pRBC was determined microscopically; OD optical density. The mean OD was determined for each isolate. Using a positivity cut-off of 3; 3D71: positive control for the first experiment. 3D72.3.…10: positive control for the remaining experiments; RBC1: negative control for the first experiment. RBC2.3.…10: negative control for the remaining experiments
  2. aPatient with convulsions
  3. bPatient with prostration