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  • Case report
  • Open Access
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The left atrial bacterial vegetative mass due to Corynebacterium striatum as a presentation of myxoma: a case report

BMC Infectious DiseasesBMC series – open, inclusive and trusted201717:368

https://doi.org/10.1186/s12879-017-2468-8

  • Received: 1 October 2016
  • Accepted: 16 May 2017
  • Published:
Open Peer Review reports

Abstract

Background

Corynebacterium striatum is a member of the non-diphtherial corynebacteria, which are ubiquitous in nature and generally colonize the skin and mucous membranes of humans. Rarely, it causes infective endocarditis (IE). We report a case of rare left atrial bacterial vegetative mass due to C. striatum masquerading as a myxoma identified through a tortuous diagnostic process, and present a brief review of the relevant literature.

Case presentation

We present a case of 63-year-old man who presented with progressively worsening dyspnea on exertion and lower leg edema, and was diagnosed with heart failure. Transesophageal echocardiography (TEE) revealed that the left atrium was filled with a 2.7 cm × 2.6 cm mass. The patient, who had no signs of infection or related risk factors, was suspected of having a left atrial myxoma clinically. After excising the mass, the histopathology suggested thrombus with no myxocytes. Postoperatively, a fever appeared and C. striatum was isolated from the blood cultures. Although antibiotics were used, the symptoms of heart failure worsened gradually and echocardiography revealed valve vegetation. The patient underwent a second operation because of IE. Surprisingly, the mass was confirmed to be a bacterial vegetation due to C. striatum based on Gram staining at a 1000× magnification, although this was not noted on routine pathological examination of the two surgical specimens.

Conclusions

Physicians should be aware of Corynebacterium in blood cultures, which cannot simply be assumed to be a contaminant. A diagnosis of IE should be suspected, particularly in high-risk patients or those with an unexplained fever. Our patient had IE due to C. striatum with no risk factors. This case supports the diagnosis of IE using a combination of pathology and etiology.

Keywords

  • Infective endocarditis
  • Corynebacterium Striatum
  • Atrial myxoma
  • Bacterial vegetation
  • Case report

Background

Infective endocarditis (IE) is a lethal disease that has undergone major changes in both host and pathogens over the past 20 years [1]. The clinical presentation includes a fever, new or changing heart murmur, embolic phenomena [2], heart failure, dyspnea, splinter hemorrhages, Roth spots, and glomerulonephritis. However, atypical clinical presentations of IE are common in the elderly or immunocompromised patients [3]. Staphylococcus aureus is the most common cause of IE in most of the industrialized world, whereas Corynebacterium striatum is a very rare cause.

Here, we present a patient with a left atrial bacterial vegetative mass due to C. striatum mimicking a myxoma, who was treated successfully with combined medical and surgical methods after a tortuous diagnostic process.

Case presentation

A 63-year-old man with a history of hypertension and atrial fibrillation presented with progressively worsening dyspnea on exertion and aggravated lower leg edema for 1 month without a fever, coughing, or sore throat. He had no history of surgery. The symptoms of heart failure markedly improved after a 2-week treatment with diuretics in a local hospital. However, echocardiography showed a mobile mass in the left atrium; therefore, the patient was transferred to our hospital for further treatment. On admission, his vital signs were blood pressure of 145/90 mmHg, atrial fibrillation with a ventricular rate of 81 beats per min, and a body temperature of 36.3 °C. Laboratory tests showed a white blood cell (WBC) count of 3.8 × 103/μL (reference range 4.0–10.0 × 103/μL) with 44.7% neutrophils, and a serum hypersensitive C-reactive protein (hs-CRP) level of 8 mg/L (reference range 0.8–8 mg/L). His urine and feces examinations were normal. Transthoracic echocardiography (TTE) revealed a 2.7 cm × 2.6 cm left atrial mass that was suspected of being a myxoma (Fig. 1a). TEE also revealed a 2.7 cm left atrial mass attached by a thin stalk (0.15 cm) to the base of the atrial apex, swinging freely within the left atrial cavity (Fig. 1b and Additional file 1). The patient was managed surgically. The mass was confirmed to originate from the atrial apex and appeared grossly myxoid and fragile. Surprisingly, however, the histopathology of the specimen showed no characteristic stellate mesenchymal cells to support the diagnosis of myxoma. Instead, it revealed a fibrinous exudate and chronic inflammation with a few mononuclear cells and a macrophage reaction (Fig. 2a). Therefore, the mass was considered to be a left atrial thrombus.
Fig. 1
Fig. 1

a Transthoracic echocardiography and (b)transesophageal echocardiography showing a 2.7 cm × 2.6 cm hypoechoic mass (arrow) attached by a thin stalk (measuring 0.15 cm in size) to the base of the atrial apex, freely swing within the left atrial cavity

Fig. 2
Fig. 2

Pathological findings and staining of bacteria. a Hematoxylin and eosin (H&E) stain of the first excised mass showing large numbers of fibrinous exudate (double →) and chronic inflammation with a few mononuclear cells (→) and macrophage (→) (×200). b Gram’s staining of blood cultures showing large numbers of C. striatum (×1000). c Gram’s staining of the second excised vegetation showing Corynebacterium (×1000). d Gram’s staining and (e)Wright’s staining of the first excised mass for Corynebacterium (×1000). f Corynebacterium can not be found in second excised vegetation by Gram’s staining (×400)

Additional file 1: Video that demonstrates a 2.7 cm left atrial mass attached by a thin stalk to the base of the atrial apex, which was extremely similar to the myxoma, freely swing within the left atrial cavity, 7 s, 6.8 MB. (MP4 6644 kb)

Despite the preventive use of cefuroxime sodium (1500 mg every 12 h), his temperature increased to 39.5 °C on the fourth day. The WBC count increased to 9.5 × 10 [3]/μL with 85.9% neutrophils and his hs-CRP level rose to 183.9 mg/L. Four sets of blood cultures were drawn. Urgent computed tomography (CT) of the chest showed bilateral pulmonary infiltrates with sputum culture positive for Acinetobacter baumannii. Consequently, cefoperazone, sulbactam, and fosfomycin were started. Nevertheless, his fever recurred for 2 weeks. The blood cultures yielded C. striatum (Fig. 2b) that was resistant to ceftriaxone, penicillin, meropenem, and clindamycin and sensitive to vancomycin. Instead of vancomycin, linezolid was chosen to treat the C. striatum bacteremia after considering the elevated creatinine level of 117 μmol/L (reference range 59–104 μmol/L). However, the symptoms of heart failure worsened gradually and echocardiography revealed valve excrescence and severe aortic insufficiency. Therefore, the patient underwent surgical management and treatment with 500 mg daptomycin daily. A double valve replacement was performed. At surgery, a 1 cm × 1 cm vegetation was seen at the left coronary cusp of the aortic valve, with perforation of the right coronary cusp of the aortic valve and moderate mitral regurgitation. The fever subsided rapidly and six sets of blood cultures obtained postoperatively were negative. Histological examination of the new vegetation indicated large numbers of Corynebacterium on Gram staining (Fig. 2c) and vegetation cultures confirmed that the bacterium was C. striatum. We also found the same Corynebacterium on the first surgical specimen using Gram and Wright’s staining (Fig. 2e). We speculate that the left atrial mass was an occult bacterial embolus and large amounts of bacteria entered the blood stream and induced the valve damage after the first operation. Subsequently, the patient received a 4-week course of intravenous daptomycin and was switched to oral linezolid for 3 weeks. The patient’s general condition markedly improved and he was discharged from our hospital.

Discussion

IE is a potentially lethal disease with a low incidence (1.7–7.9 cases/100,000 inhabitants). C. striatum is a rare cause of IE, causing about 0.33% of all cases of IE [4]. As a member of the corynebacteria, C. striatum is a Gram-positive, aerobic, non-sporulating bacillus that grows slowly in cultures and is distributed in the skin and mucous membranes of normal hosts and hospitalized patients. It is one of the more commonly isolated coryneform bacteria in the clinical microbiology laboratory and is usually considered a contaminant because of its low virulence. However, C. striatum can cause not only IE but also a variety of different infections such as pneumonia, empyema, peritonitis, arthritis, keratitis, intrauterine infections, wound infection, breast abscess, and osteomyelitis [5]. Risk factors for Corynebacterium endocarditis include pre-existing cardiac disease, a history of bacterial endocarditis, and the presence of prosthetic devices [6]. Rufael et al. [7] reported the first case of native valve endocarditis due to C. striatum, which required a combination of medical and surgical treatments [7]. Of the 24 cases of C. striatum endocarditis found in PubMed (Table 1) [630], most showed a predilection for heart valves. In our case, however, C. striatum colonized the left atrial apex masquerading as a left atrial myxoma instead of attaching to the heart valves. Looking back on our data, there were three main potential causes of misdiagnosis. First, the patient had no classic symptoms of IE on admission. Second, both TEE and the surgical findings supported the diagnosis of left atrial myxoma. Third, the initial histopathology was misleading.
Table 1

Summarizing previously reported cases of C. striatum endocarditis

Reference

Age

Sex

Associated illness

Valve

Intervention

Outcome

8

76

M

None

Aortic

Medical

Died

7

54

M

Hypertension

Aortic

Medical and surgical

Survived

9

73

M

Pacemaker

Tricuspid

Medical and surgical

Survived

10

24

M

Ventricular shunt

Pulmonary

Medical

Survived

11

68

M

Hypertension

Mitral

Medical

Survived

12

72

F

Prosthetic valve

Aortic

Medical

Died

13

62

F

Prosthetic valve

Aortic

Medical

Survived

14

50

M

Mycotic aneurysm

Aortic

Medical and surgical

Survived

15

61

F

Rheumatic fever

Mitral

Medical

Survived

15

72

F

Prosthetic valve

Mitral

Medical

Survived

16

46

F

Hemodialysis

Tricuspid

Medical

Survived

17

68

M

Prosthetic valve

Mitral

Medical

Survived

18

69

F

Endometrial cancer

Mitral

Medical and surgical

Survived

19

77

F

None

Mitral

Medical

Survived

6

62

M

Hypertension

Aortic

Medical and surgical

Survived

20

73

F

Hypertension, chronic kidney disease, and diabetes mellitus

Mitral

Medical

Survived

21

83

M

Metastatic prostate cancer

Mitral

Medical

Died

22

71

M

Diabetes mellitus

Mitral

Medical and surgical

Died

23

71

F

Pacemaker

prosthetic valve

Mitral

Medical and surgical

Survived

24

62

M

Cardiomyopathy, diabetes mellitus and osteomyelitis

Aortic

Medical and surgical

Survived

25

69

F

ANCA+ vasculitis

Mitral

Medical and surgical

Died

26

51

M

Pacemaker

Not described

Medical and surgical

Survived

27

56

M

Diabetes mellitus, chronic kidney disease, and osteomyelitis

Mitral

Medical and surgical

Died

28

78

M

Chronic kidney disease, diabetes mellitus and pacemaker

Tricuspid and right ventricular wall

Medical and surgical

Survived

29

53

F

None

Quadricuspid aortic

Medical and surgical

Survived

The category “definite IE based on clinical criteria” involves with at least two major criteria, or one major criterion and three minor criteria, or five minor criteria. Major criteria include blood culture positive for IE, evidence of endocardial involvement, echocardiogram positive for IE, and new valvular regurgitation. According to the clinical, echocardiographic and biological findings, as well as the results of serologies, the patient did not meet the modified Duke’s criteria for diagnosis of endocarditis when he was transferred to our hospital. However, two of the major Duke criteria were met after the first operation―the positive blood culture and echocardiographic findings―enabling a definitive diagnosis [31]. Looking back, Corynebacterium was present in the first surgical specimen in our case. Therefore, the left atrial mass should have been considered an occult bacterial vegetation. The C. striatum was likely completely surrounded by fibrous tissue, so the patient had no signs of infection until bacteria were released by the first surgery. Ori Elkayam et al. [22] pointed out that IE is the most common manifestation of C. striatum, particularly in patients with nosocomial risk factors. But it is a potential pathogen even in normal hosts with no risk factors, such as our patient. Therefore, in addition to the routine pathological examination, special stains such as Gram staining or Wright’s staining should be performed if a few macrophages are seen. When a patient with suspected myxoma develops an unmanageable fever postoperatively, physicians should be alert to the progress of IE and evaluate the possibility with a combined pathological examination and blood cultures. The pathological examination of resected tissue or embolic fragments remains the gold standard for the diagnosis of IE. Nevertheless, we fell into a trap in this case because no pathogens were found in the first or second surgical specimens on examination with a medical microscope at a 400× magnification (Fig. 2f). We confirmed that C. striatum in positive blood cultures was responsible for the IE and that this diagnosis was supported by the results of Gram staining and Wright’s staining of tissue specimens viewed at a 1000× magnification with an oil immersion lens. Daptomycin is an effective drug for C. striatum and has been used in some patients with endocarditis caused by this organism [28]. Combined antibiotic treatment and surgery were performed because of the uncontrolled infection and severe aortic regurgitation based on the ESC guidelines [32]. The patient had a slow, uneventful recovery.

Conclusions

Corynebacterium in positive blood cultures cannot simply be assumed to be a contaminant. The diagnosis of IE should be suspected, particularly in high-risk patients or those with an unexplained fever. Gram staining can provide additional support for a diagnosis of IE, particularly when the pathological examination implies an inflammatory response, and we should consider the size of the pathogenic bacteria and select the appropriate magnification when examining slides. This can guide postoperative antibiotic use and reduce the risk of a second surgery. In addition, it is necessary to make a suitable standard analysis protocol of cardiac masses to include thorough microbiological analysis, which can reduce misdiagnosis and improve the standard of care.

Abbreviations

C. striatum

Corynebacterium striatum

CT: 

Computed tomography

hs-CRP: 

Hypersensitive C-reactive protein

IE: 

Infective endocarditis

TEE: 

Transesophageal echocardiography

TTE: 

Transthoracic echocardiography

WBC: 

White blood cell counts

Declarations

Acknowledgements

None.

Funding

Not applicable.

Availability of data and materials

All data contained within the manuscript.

Authors’ contributions

Jun Xu was responsible for the acquisition of data and drafted the manuscript; Xia Zheng revised it critically for important intellectual content and gave final approval to the version to be published. Qing Yang and Jun Li were responsible for Lab test and histologic examination. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Written informed consent was obtained from the patient for publication of this case report and the accompanying images.

Ethics approval and consent to participate

Not applicable.

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Authors’ Affiliations

(1)
Intensive Care Unit, The First Affiliated Hospital, College of Medicine, Zhejiang University, 79 Qingchun Road, Hangzhou, 310003, People’s Republic of China
(2)
Department of Clinical Laboratory, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People’s Republic of China
(3)
Department of Pathology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People’s Republic of China

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Copyright

© The Author(s). 2017

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