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Table 1 Primers sequence and PCR conditions for molecular characterization of microscopy-positive and serology-positive samples

From: Determining intestinal parasitic infections (IPIs) in inmates from Kajang Prison, Selangor, Malaysia for improved prison management

Species PCR reaction Primers set Primers sequence (5’-3’) PCR conditions Total cycles (2–4)f Product size Reference
     1a 2b 3c 4d 5e    
Blastocystis sp.   Blast 505–532 F-GGA GGT AGT GAC AAT AAA TC 95 °C, 4 min 95 °C, 30 s 54 °C, 30 s 72 °C, 30 s 72 °C, 5 min 35 500 bp Böhm-Gloning et al., 1997
  Blast 998–1017 R TGC TTT CGC ACT TGT TCA TC Santin et al., 2011
S. stercoralis Primary SS–FO F-ATC CTT CCA ATC GCT GTT GT 94 °C, 5 min 94 °C, 45 s 58 °C, 60 s 72 °C, 1 min 72 °C, 5 min 35   Nilforoushan et al., 2007
  SS–RO R-TTT CGT GAT GGG CTA ATT CC
Secondary SS–FI F-GTA ACA AGG TTT TCG TAG GTG A 94 °C, 2 min 94 °C, 45 s 60 °C, 45 s 72 °C, 1 min 72 °C, 5 min 30 680 bp  
  SS–RI R-ATT TAG TTT CTT TTC CTC CGC TT  
Entamoeba Primary E-1 F-TAA GAT GCA CGA GAG CGA AA 96 °C, 2 min 92 °C, 1 min 56 °C, 1 min 72 °C, 1 min 30 s 72 °C, 7 min 30   Khairnar & Parija, 2007
   E-2 R-GTA CAA AGG GCA GGG ACG TA         
E. histolytica Secondary EH-1 F-AAG CAT TGT TTC TAG ATC TGA G 96 °C, 2 min 92 °C, 1 min 48 °C, 1 min 72 °C, 1 min 30 s 72 °C, 7 min   439 bp  
   EH-2 R-AAG AGG TCT AAC CGA AAT TAG         
E.dispar   ED-1 F-TCT AAT TTC GAT TAG AAC TCT        174 bp  
   ED-2 R-TCC CTA CCTATT AGA CAT AGC         
E. moshkovskii   Mos-1 F-GAA ACC AAG AGT TTC ACA AC        553 bp  
   Mos-2 R-CAA TAT AAG GCT TGG ATG AT         
        Reference
         5    
C. parvum Primary N-DIAGF2 F-CAA TTG GAG GGC AAG TCT GGT GCC AGC 95 °C, 5 min 94 °C, 30 sec 68 °C, 1 min 72 °C, 30 sec 72 °C, 10 min 35 655 to 667 bp Nichols et al., 2003
   N-DIAGR2 R-CCT TCC TAT GTC TGG ACC TGG TGA GT         
  Secondary CPB-DIAGF F-AAG CTC GTA GTA GTT GGA TTC TG 95 °C, 5 min 94 °C, 30 sec 60 °C, 1 min 72 °C, 30 sec 72 °C, 10 min   435 bp Johnson et al., 1995
   CPB-DIAGR R-TAA GGT GCT GAA GGA GTA AGG         
  1. aInitial denaturation
  2. bDenaturation
  3. cAnnealing
  4. dExtension
  5. eFinal extension
  6. fTotal cycles only applicable for PCR conditions 2–4