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Outcome correlation of smear-positivity but culture-negativity during standard anti-tuberculosis treatment in Taiwan
© Chao et al.; licensee BioMed Central. 2015
Received: 24 September 2014
Accepted: 2 February 2015
Published: 18 February 2015
The appearance of smear-positivity but culture-negativity (SPCN) for acid-fast bacilli among sputum specimen is frequently found in pulmonary tuberculosis (TB) patients during treatment. This study aimed to investigate clinical risk factors, impacts on treatment course, and relapse pattern associated with sputum SPCN.
We retrospectively enrolled 800 patients with culture-proven pulmonary TB who were receiving standard treatment and follow-up at six TB-referral hospitals in Taiwan between January 2006 and December 2007. Relevant patient characteristics and chemotherapy data were analyzed for associations with incidence of SPCN. Data from patients who relapsed within 3 years after completing treatment were analyzed for associations with SPCN during treatment.
Of the 800 subjects, 111 (13.8%) had sputum SPCN during treatment. Three factors were found to predict the development of SPCN; namely, high initial acid-fast staining grading (OR, 3.407; 95% CI, 2.090–5.553), cavitation on chest-X ray films (OR, 2.217; 95% CI, 1.359–3.615), and smoking (OR, 1.609; 95% CI, 1.006–2.841). Patients with SPCN had longer treatment duration (rifampicin: 284 ± 91 vs. 235 ± 69 days, P <0.001; isoniazid: 289 ± 90 vs. 234 ± 69 days, P < 0.001) than those without SPCN. Finally, the rate of relapse within 3 years of completing treatment was similar for groups with/without SPCN (2.7%, 3/111 vs. 1.0%, 7/689, respectively; P = 0.15).
In conclusion, severity of infection was a major risk factor for SPCN during treatment; however, the relapse rate within 3 years of completing treatment was not affected by the appearance of SPCN.
Tuberculosis (TB) remains a leading health problem worldwide. The causal pathogen Mycobacterium tuberculosis (Mtb) has extraordinarily adaptive strategies for different stressful circumstances, including anti-mycobacterial treatment [1,2]. Because smear-positivity for acid-fast bacilli in sputum specimens indicates a high mycobacterial load, culture-positivity for mycobacteria is expected in most cases. However, during antimycobacterial treatment, smear-positivity for acid-fast bacilli may not equate with culture-positivity for mycobacteria. Two recent studies have reported culture-negativity with smear-positivity at the end of the second month of intensive treatment in 44.2% of patients (50/113) in Taiwan and 46.45% (40/86) in Cameroon [3,4]. Additionally, in our previous study investigating 111 patients with smear-positivity in the fifth month of treatment, we found that 71.6% of cases (83/111) had smear-positive but culture-negative (SPCN) sputum . Given that treatment failure is currently defined as positive sputum smear or culture in the fifth month or later of treatment, it is potentially risky to assess treatment failure by sputum smear alone because of the possibility of SPCN . Such SPCN phenomena have great clinical impact in the long period of waiting for culture results of smear-positive sputum samples. Uncertainty about culture results may lead to prolonged isolation and suspicion of treatment failure [7,8]. Therefore, longitudinal studies are required to investigate the outcomes of SPCN phenomenon during treatment. In this study, we aimed to investigate risk factors, including severity of TB and clinical predisposing factors for TB, and their impacts on treatment and relapse of patients with sputum SPCN.
This study was conducted retrospectively in six TB-referral hospitals in Taiwan. The Directly Observed Therapy (DOT) program was launched in Taiwan in April 2006 and DOT coverage of smear positive cases reached 92.6% in 2007 .
Cases of relapse were also identified by reviewing the databases of the CDC; and diagnoses of relapse were based on both chest-X ray films (CXR) and adequate sputum mycobacterial evidence. Sputum SPCN was defined as sputum that was smear-positive for acid-fast bacilli but did not yield any mycobacteria on either liquid or solid media. Our research was performed in compliance with the Helsinki Declaration and approval was obtained from our local ethics committee (Institutional Review Board of the Chung Shan Medical University Hospital, Reference No: CS11051).
In 2004, Taiwan CDC established the National Reference Laboratory of Mycobacteriology, which is responsible for formulating standard operating procedures and assessing and regulating the quality control programs of all laboratories in the mycobacterial laboratory examination network . Briefly, the relevant standard procedures were performed as follows. Early-morning sputum samples were decontaminated with NaOH. Ziehl–Nielsen stained smears were examined and graded according to the American Thoracic Society guidelines . As for mycobacterial culture, solid culture media, such as Lowenstein–Jensen media and 7H10 or 7H11 media, and liquid culture media, such as BacT/ALERT 3D (bioMerieux, Lyon, France) and the BACTEC MGIT (BACTEC Mycobacterium Growth Indicator Tube, MGIT 960 system, Becton-Dickinson, Sparks, MD, USA) were used. For identification, in addition to morphology and growth rate of the bacterial colonies, biochemical tests, and molecular assays were used to identify Mtb . In accordance with the tuberculosis treatment guidelines in Taiwan , all patients’ sputum smears and cultures had been checked in the second and fifth months after initiation of treatment; monthly sputum checks had also been performed until sputum conversion in all patients with positive smears.
The medical records of enrolled patients were reviewed to obtain data on associated medical conditions, laboratory findings, hemoglobin A1C at the start of anti-TB treatment, serial sputum results, treatment regimen, and outcomes. CXRs at the start of treatment were evaluated by two pulmonologists from the Chest Hospital who were blinded to the clinical data. When their interpretations differed, the image was further reviewed by a third chest specialist who was also blinded to the results.
Data are presented as the frequencies (n) or percentages (%) for categorical factors and as means ± standard deviations for continuous factors. Categorical variables were compared using the χ2 test and differences in continuous variables were analyzed using the Mann–Whitney U test. Multivariate logistic regression analysis was used to determine independent variables that predicted SPCN. The Cochran–Armitage trend test was used to assess differences in trends in acid-fast smear (AFS) grading and cavity size in patients with or without sputum SPCN. Statistical significance was set at P < 0.05, two-sided. All data were analyzed using SPSS version 16.0 (SPSS, Chicago, IL, USA).
This study was approved by the Institutional Review Board of the Chung Shan Medical University Hospital (CSMUH No: CS11051).
Characteristics of patients with and without sputum SPCN during treatment
Sputum SPCN (+)
Sputum SPCN (−)
N = 800
N = 111
N = 689
55 ± 20
54 ± 18
55 ± 21
p = 0.784
p = 0.033
Body Weight (kgs)
57 ± 11
56 ± 11
57 ± 11
p = 0.332
p < 0.001
p = 0.008
p = 0.003
9.8 ± 3.2
10.6 ± 2.8
9.6 ± 3.3
p = 0.149
Chest-X ray findings
p < 0.001
Cavity > = 4 cm
p < 0.001
p = 0.759
Sputum acid-fast stain
p < 0.001
p = 0.354
p = 0.727
p = 0.893
Body weight loss (>5%)
p = 0.468
8469 ± 6840
8758 ± 2772
8423 ± 7287
p = 0.136
13 ± 2
13 ± 2
13 ± 2
p = 0.995
3.7 ± 2.2
3.4 ± 0.6
3.8 ± 2.5
p = 0.390
1.1 ± 0.8
1.09 ± 0.5
1.15 ± 0.8
p = 0.450
Multivariate logistic regression for sputum SPCN in TB patients
OR (95% C.I.)
Age, per 1 year increment
Gender, male vs. female
Cavitation on chest X ray
2.217 (1.359 - 3.615)
High initial acid fast stain grading*
3.407 (2.090 - 5.553)
1.690 (1.006 - 2.841)
WBC (per 1 cell/dLdecrement)
Hemoglobin, (per 1 g/dLdecrement)
Treatment and outcome of patients with and without sputum SPCN
N = 111
N = 689
289 ± 90
234 ± 71
p < 0.001
284 ± 91
235 ± 69
p < 0.001
228 ± 105
191 ± 96
p = 0.001
137 ± 136
90 ± 74
p = 0.001
Streptomycin (days) (total cases)
84 ± 76 (18)
43 ± 34 (39)
p = 0.036
Levofloxacin (days) (total cases)
132 ± 141 (11)
172 ± 196 (17)
p = 0.559
Moxifloxacin (days) (total cases)
215 ± 120 (6)
162 ± 146 (13)
p = 0.456
Treatment days after final positive culture
261 ± 72
220 ± 73
p < 0.001
p = 0.001
p = 0.677
Sputum smear and culture status during treatment
After treatment for 2 months
p < 0.001*
p < 0.001*
After treatment for 5 months
p < 0.001*
p = 0.707*
After treatment for 9 months
p = 0.001*
p = 0.407*
Relapse within 3 years
p = 0.150*
Characteristics of the 10 relapse cases a
HER (194 d), Z (8 d)
HER (184 d), Z (63 d)
HER (182 d), Z (77 d)
HER (328 d), Z (17 d)
HER (252 d), Z (84 d)
HR (539 d), EZ (32 d)
HER (310d), Z (30 d)
HER (358 d), Z (12 d)
HER (343 d)
HR (231 d), E (21 d), Z (15 d)
Taken together, our data show that sputum culture conversion and relapse rates are not affected by the sputum SPCN, possibly because patients with SPCN received treatment for longer than those without sputum SPCN.
This study aimed to investigate the risk factors and clinical impact of the phenomenon of sputum SPCN. We found that severe pulmonary TB, as evidenced both by cavitation formation on CXR and high sputum AFS grading, and smoking contributed to the occurrence of sputum SPCN during treatment. Patients with sputum SPCN received continuation phase treatment for longer than patients without sputum SPCN; however, the relapse rate within 3 years after completion of treatment was similar between the two groups.
Our data showed cavitation on CXR is an important risk factor for sputum SPCN. Cavitation formation, a well-known characteristic presentation of TB infection, is believed to result from caseous granulomatous inflammation. With advances in knowledge regarding TB immunity, granuloma formation has been subdivided into highly-structured solid granulomas that contain Mtb and caseous granulomas with less-organized structures and liquefied centers, which lead to cavity formation [14,15]. Therefore, the strong correlation between cavitation and the occurrence of SPCN mycobacteria indicates that caseous granulomas, which contain numerous Mtb released from dead macrophages, may be the major source of SPCN mycobacteria. We therefore surmised that sputum SPCN may be a clinical indicator of host-pathogen interactions in the lungs during TB infection.
SPCN acid-fast bacilli were previously considered to be dead bacilli; however, accumulating evidence has now shown that culturability does not equal viability. Rather, SPCN bacilli can be viable but non-culturable (VBNC) and culturability can be restored with additional nutrient factors, including mycolic acid or proteins called resuscitation-promoting factors [16,17]. Recently, Mukamolova et al. used culture medium with and without resuscitation-promoting factors and demonstrated not only the presence of VBNC Mtb but also an increased proportion of VBNC Mtb during treatment of pulmonary TB infection . These findings provide evidence of an adaptive phenotypic switch of mycobacteria after exposure to anti-TB treatment; such phenotype switches during treatment may lead to drug resistance and relapse . In this study, we found that patients with sputum SPCN received treatment for longer after the final positive culture than patients without sputum SPCN (261 ± 72 vs. 220 ± 73, respectively; P < 0.001) (Table 3), the duration of treatment having been decided by the TB committees in each TB referral center. When faced with sputum smear-positivity near the end of the scheduled treatment course without other evidence of treatment failure, members of these committees tended to suggest continuation of treatment and follow-up of culture results rather than immediate modification of the drug regimen. The final treatment outcome and relapse rate were similar between the two groups. Importantly, the three patients who relapsed with sputum SPCN during initial treatment did not develop any new drug resistance. Therefore, our data provide evidence that continuation of treatment may be an acceptable and practical strategy for treating patients with sputum SPCN. However, more studies are needed to establish the best treatment regimen for these patients.
The long turnaround time for culture results creates several clinical dilemmas when treating patients with severe TB and sputum SPCN. Persistence of positive AFS in sputum often leads to prolonged isolation and suspicion of treatment failure [7,8]. Our data show that sputum SPCN mostly develops 157 ± 95 days after the start of treatment. Specifically, at the end of the second month, 14.5% of all patients (116/800) were smear-positive, but only 46.6% (54/116) of these smear-positive patients were culture-positive. Similarly, at the end of the fifth month, 4.4% of all patients (35/800) were smear-positive, but only 28.6% (10/35) of these smear-positive patients were culture-positive. Our data are fully consistent with those of another study conducted in Taiwan that reported 44.2% culture positivity (50/113) in 113 patients who were smear-positive at the end of the second month . The data at the end of the fifth month are also consistent with those of our previous study, which showed only 25.2% (28/113) culture positivity in patients who were smear-positive at the end of the fifth month . This longitudinal study showed that SPCN develops as early as 30 days after initiating treatment and is rarely found after 240 days of treatment (Figure 2). Therefore, AFS alone should not be used as the sole means of assessing treatment response during this period. Instead, as we showed in our previous study, multi-dimensional assessment, including culture conversion after initial phase treatment, CXR improvement, directly observed short-course therapy (DOTS), and AFS grading ≥3+ or not are crucial to thorough evaluation of treatment responses in patients with severe pulmonary TB .
Interestingly, we identified smoking as a minor risk factor for sputum SPCN in this study. Smoking, including passive smoking, has been found to be associated with active TB in Taiwan and other countries; the suppressive effect of smoking on airway defense has been assumed to be the causative factor [20-22]. Moreover, smoking was recently found to affect the rate of 2-month culture conversion during treatment . However, whether the immune-suppressive effects of smoking also contribute to the development of sputum SPCN in pulmonary TB patients remains to be clarified.
Finally, SPCN is especially important in countries with limited laboratory capacity. In Taiwan, the laboratory capacity is adequate and all sputum samples are sent for both smear and culture. However, in many countries diagnosis of TB and assessment of treatment outcome still relies on sputum smear only. In these countries, the strong possibility of SPCN during anti-TB treatment in patients with severe TB infection should be taken into account when assessing treatment failure. As we showed in our previous study, multi-dimensional assessment, quality patient supervision, and chest radiography are crucial for avoiding unnecessary modification of treatment regimens .
This study has several noteworthy limitations. First, the viability of organisms in SPCN sputum cannot be ascertained under routine standard culture conditions without adding additional resuscitation factors. Second, the standard decontamination procedures in sputum processing can decrease culturability. We believe the differential error was not caused by such an inhibitory effect on culturability in this study because all cases had positive cultures for Mtb in sputum on enrollment and the inhibitory effect on culturability was the same for all sputum samples throughout the study period. Third, we totally excluded patients with non-tuberculous mycobacteria (NTM) in this study, and only AFS-positive organisms that grew no colonies on either liquid or solid media were classified as SPCN. Therefore, we postulate that we may have underestimated the true prevalence of sputum SPCN Mtb in this study, rather than amplified it, if there was any NTM contamination.
This study provides clinical evidence of the previously ignored sputum SPCN phenomenon in patients with pulmonary TB receiving treatment. We have shown that severe TB infection, as evidenced by cavitation on CXR and high AFS grading in sputum, is the critical risk factor; smoking also contributes to the occurrence of sputum SPCN. Prolonging continuation phase treatment while awaiting results of culture of smear-positive samples appears to be a practical and safe strategy with an acceptable relapse rate; however, the ideal regimen for such patients has not yet been established.
This work was supported by Grant DOH100-DC-4-2-421 from the Institute for Biotechnology and Medicine Industry, Taiwan. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- World Health Organization: Global tuberculosis report 2013. http://apps.who.int/iris/bitstream/10665/91355/1/9789241564656_eng.pdf.
- Schnappinger D, Ehrt S, Voskuil MI, Liu Y, Mangan JA, Monahan IM, et al. Transcriptional Adaptation of Mycobacterium tuberculosis within Macrophages: Insights into the Phagosomal Environment. J Exp Med. 2003;198(5):693–704.View ArticlePubMedPubMed CentralGoogle Scholar
- Su WJ, Feng JY, Chiu YC, Huang SF, Lee YC. Role of 2-month sputum smears in predicting culture conversion in pulmonary tuberculosis. Eur Respir J. 2011;37(2):376–83.View ArticlePubMedGoogle Scholar
- Pefura-Yone EW, Kengne AP, Kuaban C. Non-conversion of sputum culture among patients with smear positive pulmonary tuberculosis in Cameroon: a prospective cohort study. BMC Infect Dis. 2014;14:138.View ArticlePubMedPubMed CentralGoogle Scholar
- Chien JY, Chen YT, Shu CC, Lee JJ, Wang JY, Yu CJ, et al. Outcome correlation of smear-positivity for acid-fast bacilli at the fifth month of treatment in non-multidrug-resistant TB. Chest. 2013;143(6):1725–32.View ArticlePubMedGoogle Scholar
- World Health Organization: Definitions and reporting framework for tuberculosis 2013. [http://apps.who.int/iris/bitstream/10665/79199/1/9789241505345_eng.pdf]
- Al-Moamary MS, Black W, Bessuille E, Elwood RK, Vedal S. The significance of the persistent presence of acid-fast bacilli in sputum smears in pulmonary tuberculosis. Chest. 1999;116(3):726–31.View ArticlePubMedGoogle Scholar
- Horne DJ, Johnson CO, Oren E, Spitters C, Narita M. How soon should patients with smear-positive tuberculosis be released from inpatient isolation? Infect Control Hosp Epidemiol. 2010;31(1):78–84.View ArticlePubMedPubMed CentralGoogle Scholar
- Hsu CBLH, Lee CY, Yang SL, Wang KF, Yang SY. Preliminary Evaluation of Taiwan’s Tuberculosis DOTS Strategy. Taiwan Epidemiol Bull. 2008;24(3):230–53.Google Scholar
- Jou R, Chiang CY, Yu CY, Wu MH. Proficiency of drug susceptibility testing for Mycobacterium tuberculosis in Taiwan. Int J Tuberc Lung Dis. 2009;13(9):1142–7.PubMedGoogle Scholar
- American Thoracic Society and the Centers for Disease Control and Prevention. Diagnostic Standards and Classification of Tuberculosis in Adults and Children. Am J Respir Crit Care Med. 2000;161(4 Pt 1):1376–95.Google Scholar
- Ting-Fang Wang RJ. Quality Management of Mycobacteriology Laboratory in Taiwan: Current Status and Future Perspectives. Taiwan Epidemiol Bull. 2012;28(18):278–85.Google Scholar
- Taiwan Centers for Disease Control. Taiwan guidelines for tuberculosis diagnosis and treatment. 2006.Google Scholar
- Ramakrishnan L. Revisiting the role of the granuloma in tuberculosis. Nat Rev Immunol. 2012;12(5):352–66.PubMedGoogle Scholar
- Kaufmann SH, Dorhoi A. Inflammation in tuberculosis: interactions, imbalances and interventions. Curr Opin Immunol. 2013;25(4):441–9.View ArticlePubMedGoogle Scholar
- Balaban NQ, Merrin J, Chait R, Kowalik L, Leibler S. Bacterial persistence as a phenotypic switch. Science. 2004;305(5690):1622–5.View ArticlePubMedGoogle Scholar
- Baek SH, Li AH, Sassetti CM. Metabolic regulation of mycobacterial growth and antibiotic sensitivity. PLoS Biol. 2011;9(5):e1001065.View ArticlePubMedPubMed CentralGoogle Scholar
- Mukamolova GV, Turapov O, Malkin J, Woltmann G, Barer MR. Resuscitation-promoting factors reveal an occult population of tubercle Bacilli in Sputum. Am J Respir Crit Care Med. 2010;181(2):174–80.View ArticlePubMedGoogle Scholar
- Levin BR, Rozen DE. Non-inherited antibiotic resistance. Nat Rev Microbiol. 2006;4(7):556–62.View ArticlePubMedGoogle Scholar
- Lin HH, Ezzati M, Chang HY, Murray M. Association between tobacco smoking and active tuberculosis in Taiwan: prospective cohort study. Am J Respir Crit Care Med. 2009;180(5):475–80.View ArticlePubMedGoogle Scholar
- Patra S, Sharma S, Behera D. Passive smoking, indoor air pollution and childhood tuberculosis: a case control study. Indian J Tuberc. 2012;59(3):151–5.PubMedGoogle Scholar
- Feldman C, Anderson R. Cigarette smoking and mechanisms of susceptibility to infections of the respiratory tract and other organ systems. J Infect. 2013;67(3):169–84.View ArticlePubMedGoogle Scholar
- Maciel EL, Brioschi AP, Peres RL, Guidoni LM, Ribeiro FK, Hadad DJ, et al. Smoking and 2-month culture conversion during anti-tuberculosis treatment. Int J Tuberc Lung Dis. 2013;17(2):225–8.View ArticlePubMedPubMed CentralGoogle Scholar
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