- Research article
- Open Access
- Open Peer Review
The role of Sequence Type (ST) 131 in adult community-onset non-ESBL-producing Escherichia colibacteraemia
© Wu et al.; licensee BioMed Central Ltd. 2014
- Received: 31 May 2014
- Accepted: 22 October 2014
- Published: 7 November 2014
To compare the epidemiological and clinical features and outcome in clonal group O25b/ST131 and non-clonal group O25b/ST131 in adult patients with non-extended-spectrum B-lactamase (ESBL)-producing Escherichia coli (E. coli) bacteraemia.
We collected 371 consecutive isolates with community-onset non-ESBL producing E. coli bloodstream infection in 2010 in a 1200-bed hospital in Taiwan. Twenty adult patients with clonal group O25b/ST131 and 40 patients with non-clonal group O25b/ST131 were compared.
Clonal group O25b/ST131 accounted for 5.9% of total isolates. The underlying disease and healthcare-associated risk factors were similar in the case and control groups. Patients with the clonal group O25b/ST131 were less likely to have intra-abdominal infection (0% vs. 22.5%; p < 0.05) than patients from the control group. The Day 30 mortality rate was similar in the case and control groups (15% vs. 12.5%).
Clonal group O25b/ST131 was found in both multidrug-resistant and susceptible E. coli strains, causing community-onset bloodstream infection. Although O25b/ST131 does not lead to a higher mortality than other isolates, choosing an appropriate antimicrobials in the empirical therapy of community-onset E. coli bacteraemia has become more challenging.
- E. coli
- Community onset
- Fluoroquinolone resistant
- ST (sequence type) 131
From 2000 to 2006, the Escherichia coli clone O25:H4-ST131, which produces CTX-M-15 extended-spectrum B-lactamase (ESBL), was identified in three continents -. ST131's distinctive combination of resistance and virulence, as well as its widespread dissemination among the locals, may underlie its epidemiologic success. Later studies have showed that clone ST131 exists in non-ESBL producing fluoroquinolone-resistant E. coli isolates -. ST131 accounted for 25% to 78% of fluoroquinolone resistant E. coli infection in the surveillance form in Asia and the US -. In our previous studies, fluoroquinolone (29%) and cefazolin (25%) resistant uropathogens were common in adults with community-onset urinary tract infections (UTI) in a teaching hospital in southern Taiwan.  There were several risk factor to be independently associated with community onset UTI fluoroquinolone resistant pathogens (i.e. recent hospitalization, underlying old stroke, and diabetes mellitus) . Although cephalosporin and fluoroquinolone are the recommended antimicrobials to use in community-onset complicated UTI or bloodstream infection, the emergence of clone ST131 with fluoroquinolone resistance in acute pyelonephritis poses a challenge in choosing the adequate antimicrobials. In our institution, we often used 3rd generation cephalosporin such as ceftriaxone or fluoroquinolone such as levofloxacin or ciprofloxacin as empirical antibiotics for community-onset complicated UTI or gram negative bacillary bacteremia.
Our previous studies compared the clinical features and outcome in ST131 vs. non-ST131 in ESBL-producing E. coli bacteremia . We found that ST131 was associated with non-catheter related UTI (urinary tract infection) and that it is not associated with higher mortality . According to our review of the literature, we did not find clinical data for clone ST131 in community-onset ESBL-negative E. coli bacteremia. To further understand the role of ST131 in community-onset non-ESBL-producing E. coli bacteremia, we collected non-duplicated consecutive E. coli bloodstream infection isolates in 2010 in a medical center in southern Taiwan. Our goals were to determine the percentage of ST131 in community-onset non-ESBL-producing E. coli bloodstream infection and to find out if patients with ST131 clone have clinical features or outcome different from patients with a non-ST131 clone.
Study design, setting and participants
In the present study, non-susceptibility arbitrarily refers to the category of intermediate and resistant results, obtained using the MIC (minimal inhibitory concentration) value in accordance with the criteria from the Clinical and Laboratory Standards Institute, M100-S21.
Variables, collection of data and definitions
All patients were evaluated using a standard case recording form. Each clinical course of infection was evaluated and recorded according to the information supplied by primary care physicians and medical records reviewing retrospectively. The diagnosis of infection focus of bacteremia was based on clinical, bacteriological, and radiological investigations.
The following items were recorded for each patient: age; sex; underlying illness; infection syndrome type; history of hospitalization within the previous three months; antimicrobials exposure within the previous three months, existence of a urinary catheter; initial empirical antimicrobial agents; and outcome. If an in vitro active antimicrobial agent was not administered before the final result of blood culture, the procedure was considered a discordant empirical therapy.
This study was supported by the E-Da Hospital Research Committee and approved by the institutional review board of E-Da Hospital. (EDAH IRB No./Protocol No: EMRP-102-048). Because the study was done via medical chart reviewing retrospectively and the pathogens were collected from the stock of the infectious isolates from the laboratory. The IRB waived informed consent requirements due to the research involves no more than minimal risk to the subjects and the waiver will not affect adversely the rights and welfare of the patients.
O25 positve E. coli isolates were determined by the PCR method described by Clermont et al . All O25b positive E. coli isolates were characterized by multilocus sequence typing (MLST) using the standard seven housekeeping genes (adk, fumC, gyrB, icd, mdh, purA, and recA), according to the protocol and primers specified by the E. coli MLST web site (http://mlst.warwick.ac.uk/mlst/dbs/Ecoli) , MLST was also performed on a randomly selected subset of 40 O25b negative E-coli isolates. Clonal relationships were established by pulsed-field gel electrophoresis (PFGE) of XbaI-digested genomic DNA. To compare the band patterns of aggregated data, the GelCompar software package (version 6.0; Applied Maths, Bionumerics) was used. Strains showing 80% or greater banding pattern similarity were considered to have similar or identical electrokaryotypes.
Descriptive statistics, including means, standard deviations, and ranges, were used to analyze continuous variables, whereas percentages and confidence intervals were used to analyze categorical variables. Independent-t test was used for continuous variables. A Chi-square test or Fisher's exact test was used for categorical variables. The variables in bivariate analyses with a p value <0.1 were included in a multivariate analysis, which was performed using a logistic regression model to identify factors that independently and significantly affected the outcome. A p value <0.05 was considered to be statistically significant. All statistical analyses were done using SPSS 15 for Windows, Chicago, IL, USA.
Of the 371 non-duplicated consecutive E. coli bloodstream infection isolates, 43.9%, 30.7%, 15.4%, 15.4%, 13.2%, and 4.6% were resistant to trimethoprim/sulfamethoxazole (TMP/SMZ), amoxicillin/clavunate, cefazolin, gentamicin, ciprofloxacin, and cefotaxime, respectively (data not shown). The antibiotic resistance of all 371 isolates of clonal group O25b/ST131 and non clonal group O25b/ST131 were shown in the Additional file 1: Table S1. Clonal group 131 were more likely to have TMP/SMZ, ciprofloxacin, cefmetazole, and gentimicin resistance (P < 0.05). Clonal group O25b/ST131 (n = 22) accounted for 5.9% of the total isolates. Two cases of O25-ST131 did not have a complete history in our hospital and were excluded from further clinical analysis.
Clinical features and epidemiology data in cases with clonal group O25b/ST131 and control group
Non clonal group O25b/ST131
Clonal group O25b/ST131
N = 40
N = 20
67.0 + -13.9
Renal structure abnormality
Chronic renal disease
Healthcare-associated risk factor
Hospitalization in previous 6 months
Antimicrobials exposure in 3 months
With Foley catheter
Urinary tract infection
Length of stay >2 weeks
Day 30 mortality
Univariate and multivariate analysis in risk factor of Day 30 mortality
Crude OR (95% CI)
Adjusted OR (95% CI)
Chronic renal disease
Urinary tract infection
7.0 (1.4 -34.2)
Clonal group O25b/ST131
Percentage of antibiotic resistance in ST 131 clones and nonST131 clones (N = 60)
N = 20
N = 40
We have shown that O25-ST131 is also the important clone in non-ESBL-producing E. coli bacteremia. Clonal group O25b/ST131 accounted for 5.9% in total isolates and had a higher proportion in gentamicin, TMP/SMZ and fluoroquinolone resistant isolates. Clonal group O25b/ST131 existed in both multidrug-resistant and susceptible E. coli strains causing community-onset bloodstream infection. Most cases with clonal group O25b/ST131 did not have apparent healthcare associated risk factors. This finding answers a previous suggestion that the emerging of clonal group O25b/ST131 may be explained by the enhanced ability to cause extraintestinal infection rather than selection by antimicrobial agents used in hospitals. Current study excluding ESBL positive isolates showed approximately one fourth of fluoroquinolone resistant E. coli bacteremia was O25-ST131. Similar to a previous study, although more present in multidrug-resistant isolates, ST131 was found in both antimicrobials susceptible and resistant blood isolates ,. In a bacteremia study in San Francisco, ST131 accounted for approximately 20% of all drug-resistant and susceptible isolates . Uchida et al. investigated 219 fluoroquinolone-resistant E. coli strains in Asian and found that 32% strains were from the serogroup O25 . In Korea, 25% of community-onset urine E. coli isolates were ST131 from November 2006 to August 2007 .
Similar to a previous study, O25-ST131 was less likely to be associated with intra-abdominal infection and more likely to be related to UTI . In a study from Spain, some healthcare associated risk factors such as diabetes, bedridden status, and antimicrobials exposure were independent risk factors for clone ST131 . A trend for fluoroquinolone exposure in clonal group ST131/O25b was observed in our study but did not reach statistical significance. However, the other correlations were not found in our series, which is possibly explained by the fact that we included only community-onset bacteremia, and patients with some healthcare-associated risk factors may have been excluded. Similar to the study in Spain, mortality was similar between the ST131 and non-ST131 groups . Our study is also in agreement with a previous murine sepsis model in which neither ST131 status nor fluoroquinolone resistance correlated with mortality . More cases of clone ST131 had a higher chance of ESBL E. coli infection in the following two years than cases of non-ST131. It remains to be clarified if this phenomenon is related to the colonization and transmission features of ST 131. Nevertheless, a study from France showed that ST131 had high intestine colonization and urinary tract infection abilities, and according to a recent report, ST131 clone was associated with recurrent or persistent urinary tract infection ,.
In our study, despite the high percentage use of discordant antimicrobials in both the ST131 and non-ST131 groups, the mortality was not changed much. Taking into account the predominance of ST131 in E. coli bacteremia infection, the selection of adequate empirical antimicrobials becomes difficult. In Asia, the susceptibility rate to fluoroquinolones in E. coli was approximately 70% in the urinary isolates from the SMART study . In community-acquired bacteremic acute pyelonephritis, discordant empirical therapy was associated with a worse early clinical response and longer hospital stay than in concordant therapy -. However, choosing broad-spectrum antimicrobials such as carbapenem in this type of community-onset infection raises the concern of antimicrobials selection pressure.
This study is limited by the following factors. First, this is a single center study in Taiwan that included a moderate number of case numbers, and we do not know if the result can be generalized to other parts of the world. Second, we did not collect isolates from colonization or infection after the bacteremia episode. We ignore if the ESBL isolates cultured after infection have the same genotype as the isolates collected during the non-ESBL producing E. coli bacteremia. Third, we used the O25b PCR as a screen and confirm by MLST to identify the clonal group O25b/ST131, some of isolates of ST131 but O25b negative may be missed in this screening method. The clinical significance of clone O25b negative ST131 was not known.
Our preliminary study shows that clone O25b-ST131 isolates emerged as an important cause of non-ESBL-producing E. coli bacteremia in Taiwan. Clonal group O25b/ST131 existed in both multidrug-resistant and susceptible E. coli strains causing community-onset bloodstream infection. Although O25b/ST131 does not lead to a higher mortality than other isolates, choosing an appropriate antimicrobials in the empirical therapy of community-onset E. coli bacteremia has become more challenging due to the appearance of the ST131 clone.
YHW, JLW, CHH carried out the molecular genetic studies and drafted the manuscript. CHL and HHL participated in the design of the study and collected the bacteria isolates. MFC participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
This work was supported by research grants (EDAHP103057) from E-DA hospital. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Nicolas-Chanoine MH, Blanco J, Leflon-Guibout V, Demarty R, Alonso MP, Caniça MM, Park YJ, Lavigne JP, Pitout J, Johnson JR: Intercontinental emergence of Escherichia coli clone O25:H4'sT131 producing CTX-M-15. J Antimicrob Chemother. 2008, 61: 273-281. 10.1093/jac/dkm464.View ArticlePubMedGoogle Scholar
- Clermont O, Dhanji H, Upton M, Gibreel T, Fox A, Boyd D, Mulvey MR, Nordmann P, Ruppé E, Sarthou JL, Frank T, Vimont S, Arlet G, Branger C, Woodford N, Denamur E: Rapid detection of the O25b-ST131 clone of Escherichia coli encompassing the CTX-M-15-producing strains. J Antimicrob Chemother. 2009, 64: 274-277. 10.1093/jac/dkp194.View ArticlePubMedGoogle Scholar
- Peirano G, Pitout JD: Molecular epidemiology of Escherichia coli producing CTX-M beta-lactamases: the worldwide emergence of clone ST131 O25:H4. Int J Antimicrob Agents. 2010, 35: 316-321. 10.1016/j.ijantimicag.2009.11.003.View ArticlePubMedGoogle Scholar
- Johnson JR, Nicolas-Chanoine MH, DebRoy C, Castanheira M, Robicsek A, Hansen G, Weissman S, Urban C, Platell J, Trott D, Zhanel G, Clabots C, Johnston BD, Kuskowski MA: Comparison of Escherichia coli ST131 pulsotypes, by epidemiologic traits, 1967-2009. Emerg Infect Dis. 2012, 18: 598-607. 10.3201/eid1804.111627.View ArticlePubMedPubMed CentralGoogle Scholar
- Johnson JR, Johnston B, Clabots C, Kuskowski MA, Castanheira M: Escherichia coli sequence type ST131 as the major cause of serious multidrug-resistant E. coli infections in the United States. Clin Infect Dis. 2010, 51: 286-294. 10.1086/653932.View ArticlePubMedGoogle Scholar
- Yokota S, Sato T, Okubo T, Ohkoshi Y, Okabayashi T, Kuwahara O, Tamura Y, Fujii N: Prevalence of fluoroquinolone-resistant Escherichia coli O25:H4-ST131 (CTX-M-15-nonproducing) strains isolated in Japan. Chemotherapy. 2012, 58: 52-59. 10.1159/000336129.View ArticlePubMedGoogle Scholar
- Uchida Y, Mochimaru T, Morokuma Y, Kiyosuke M, Fujise M, Eto F, Eriguchi Y, Nagasaki Y, Shimono N, Kang D: Clonal spread in Eastern Asia of ciprofloxacin-resistant Escherichia coli serogroup O25 strains, and associated virulence factors. Int J Antimicrob Agents. 2010, 35: 444-450. 10.1016/j.ijantimicag.2009.12.012.View ArticlePubMedGoogle Scholar
- Lee MY, Choi HJ, Choi JY, Song M, Song Y, Kim SW, Chang HH, Jung SI, Kim YS, Ki HK, Son JS, Kwon KT, Heo ST, Yeom JS, Shin SY, Chung DR, Peck KR, Song JH, Ko KS: Dissemination of ST131 and ST393 community-onset, ciprofloxacin-resistant Escherichia coli clones causing urinary tract infections in Korea. J Infect. 2010, 60: 146-153. 10.1016/j.jinf.2009.11.004.View ArticlePubMedGoogle Scholar
- Wu YH, Chen PL, Hung YP, Ko WC: Risk factors and clinical impact of levofloxacin or cefazolin nonsusceptibility or ESBL production among uropathogens in adults with community-onset urinary tract infections.J Microbiol Immunol Infect 2012, Oct 11(E-Pub).,Google Scholar
- Chung HC, Lai CH, Lin JN, Huang CK, Liang SH, Chen WF, Shih YC, Lin HH, Wang JL: Bacteremia caused by extended-spectrum-β-lactamase-producing Escherichia coli sequence type ST131 and non-ST131 clones: comparison of demographic data, clinical features, and mortality. Antimicrob Agents Chemother. 2012, 56: 618-622. 10.1128/AAC.05753-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Adams-Sapper S, Diep BA, Perdreau-Remington F, Riley LW: Clonal composition and community clustering of drug-susceptible and -resistant Escherichia coli isolates from bloodstream infections. Antimicrob Agents Chemother. 2013, 57: 490-497. 10.1128/AAC.01025-12.View ArticlePubMedPubMed CentralGoogle Scholar
- Horner C, Fawley W, Morris K, Parnell P, Denton M, Wilcox M: Escherichia coli bacteraemia: 2 years of prospective regional surveillance (2010-12). J Antimicrob Chemother. 2014, 69: 91-100. 10.1093/jac/dkt333.View ArticlePubMedGoogle Scholar
- López-Cerero L, Navarro MD, Bellido M, Martín-Peña A, Viñas L, Cisneros JM, Gómez-Langley SL, Sánchez-Monteseirín H, Morales I, Pascual A, Rodríguez-Baño J: Escherichia coli belonging to the worldwide emerging epidemic clonal group O25b/ST131: risk factors and clinical implications. J Antimicrob Chemother. 2014, 69: 809-814. 10.1093/jac/dkt405.View ArticlePubMedGoogle Scholar
- Johnson JR, Porter SB, Zhanel G, Kuskowski MA, Denamur E: Virulence of Escherichia coli clinical isolates in a murine sepsis model in relation to sequence type ST131 status, fluoroquinolone resistance, and virulence genotype. Infect Immun. 2012, 80: 1554-1562. 10.1128/IAI.06388-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Vimont S, Boyd A, Bleibtreu A, Bens M, Goujon JM, Garry L, Clermont O, Denamur E, Arlet G, Vandewalle A: The CTX-M-15-producing Escherichia coli clone O25b: H4'sT131 has high intestine colonization and urinary tract infection abilities. PLoS One. 2012, 7: e46547-10.1371/journal.pone.0046547.View ArticlePubMedPubMed CentralGoogle Scholar
- Tchesnokova V, Billig M, Chattopadhyay S, Linardopoulou E, Aprikian P, Roberts PL, Skrivankova V, Johnston B, Gileva A, Igusheva I, Toland A, Riddell K, Rogers P, Qin X, Butler-Wu S, Cookson BT, Fang FC, Kahl B, Price LB, Weissman SJ, Limaye A, Scholes D, Johnson JR, Sokurenko EV: Predictive diagnostics for Escherichia coli infections based on the clonal association of antimicrobial resistance and clinical outcome. J Clin Microbiol. 2013, 51: 2991-2999. 10.1128/JCM.00984-13.View ArticlePubMedPubMed CentralGoogle Scholar
- Lu PL, Liu YC, Toh HS, Lee YL, Liu YM, Ho CM, Huang CC, Liu CE, Ko WC, Wang JH, Tang HJ, Yu KW, Chen YS, Chuang YC, Xu Y, Ni Y, Chen YH, Hsueh PR: Epidemiology and antimicrobial susceptibility profiles of Gram-negative bacteria causing urinary tract infections in the Asia-Pacific region: 2009-2010 resultsfrom the Study for Monitoring Antimicrobial Resistance Trends (SMART). Int JAntimicrob Agents. 2012, 40 (Suppl): S37-S43. 10.1016/S0924-8579(12)70008-0.View ArticleGoogle Scholar
- Lee SS, Kim Y, Chung DR: Impact of discordant empirical therapy on outcome of community-acquired bacteremic acute pyelonephritis. J Infect. 2011, 62: 159-164. 10.1016/j.jinf.2010.10.009.View ArticlePubMedGoogle Scholar
- Johnson L, Sabel A, Burman WJ, Everhart RM, Rome M, MacKenzie TD, Rozwadowski J, Mehler PS, Price CS: Emergence of fluoroquinolone resistance in outpatient urinary Escherichia coli isolates. Am J Med. 2008, 121: 876-884. 10.1016/j.amjmed.2008.04.039.View ArticlePubMedGoogle Scholar
- van der Starre WE, van Nieuwkoop C, Paltansing S, van't Wout JW, Groeneveld GH, Becker MJ, Koster T, Wattel-Louis GH, Delfos NM, Ablij HC, Leyten EM, Blom JW, van Dissel JT: Risk factors for fluoroquinolone-resistant Escherichia coli in adults with community-onset febrile urinary tract infection. J Antimicrob Chemother. 2011, 6: 6650-6656.Google Scholar
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