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Table 1 Overall results of the EQA for the serological detection of WN virus infection

From: First international diagnostic accuracy study for the serological detection of West Nile virus infection

   Sample N°  
Lab. N° Assay used #8 anti-WN #12 anti-WN #5 anti-WN #6 anti-WN #10 anti-TBE #7 anti-YF #11 anti-DEN #3 neg. #2 neg. #1 unspec. Correct results in %
2 EIA/IFAih (-)/+ (-)/+ (-)/+ -/+ - - - - - - 100.0
4 EIAih +/+ +/+ +/+ -/+ -* -* - - - - 100.0
8 EIAih +/(-) +/+ +/+ -/+ - -/e - - - - 100.0
18 EIAih +/+ +/+ +/+ -/+ - -* - - - - 100.0
29 IFAih (-)/+ (-)/+ +/+ -/+ - -* - - - - 100.0
37 EIAih + + + + - -* - - - - 100.0
5 EIA F +/+ e/+ +/+ -/+ - -/(+) - - - - 90.0
6 IFAih (-)/+ (-)/+ +/+ -/+ - -/(+) - - - - 90.0
9 IFA P (-) (+)/(+) +/+ -/(+) - -* - - - - 90.0
10 IFAih (-)/+ (-) (-)/+ -/+ - - - - - - 90.0
14 EIAih (-)/+ +/+ (-)/+ -/+ - -/(+) - - - - 90.0
16 EIA F +/+ e/+ +/+ -/+ - -/(+) - - - - 90.0
17 IFAih (-)/+ (-)/+ +/+ -/+ - -/e - - - -/(+) 90.0
20 EIA F +/+ e/+ +/+ -/+ - -/(+) - - - - 90.0
23 EIA F (-)/+ (-)/+ (-)/+ -/+ - -/(+) - - - - 90.0
35 EIA F+P e/+ e/+ e/+ -/+ - -/(+) - - - - 90.0
11 IFAih +/+ +/+ +/+ (+)/+ - -/(+) - - - (+) 80.0
13 EIA F +/+ e/+ e/+ -/+ - -/(+) (+)/- - - - 80.0
24 EIAih + (-) + (-) - - - - - - 80.0
25 EIA F (+)/+ (-)/e (+)/+ -/+ - -/(+) - - - - 80.0
33 EIAih + + + + (+) (+) - - - - 80.0
15 EIA F +/+ +/+ +/+ -/+ (+)/- -/(+) ind. - - - 77.8
30 EIAih (-) (-) + + - (+) - - - - 70.0
7 NTih (+) (+) + + - - - - - - 100.0
36 NTih e + + + - - - - - - 100.0
11 NTih + + + (+) - - - - - (+) 90.0
5 NTih + + + + - (+) (+) - - - 80.0
12 NTih + + + + (+) (+) - - - - 80.0
39 NTih (-) (-) (+) (+) - - - - - - 80.0
30 NTih (-) (-) + + - (+) - - - - 70.0
  1. WN = West Nile; TBE = tick-borne encephalitis; YF = yellow fever; DEN = dengue; neg. = negative; unspec. = unspecific. x/x = IgM/IgG result: - = correct negative result; (-) = false negative result without meaning; + = correct positive result; (+) = weak positive result; e = equivocal result; ind. = indetermined. -* = negative for WN virus, but other flavivirus detected; -# = negative for WN virus, but unspecific reaction detected. Results marked in bold mean false negative/positive results or non conclusive results which may have consequences for the decision of the clinician. Laboratory reported combined results: IgM tested by EIA and IgG tested by IFA. In other cases laboratories have reported combined results for IgM and IgG (single results).
  2. EIA: Enzyme immunoassay; IFA: Immunofluorescence assay; NT: neutralisation test. Assays marked with the index "ih" are in-house assays. Assays marked in bold are commercial assays. Manufacturers: F = Focus Diagnostics Inc., Cypress, CA; USA, P = PanBio Ltd., Queensland, Australia.