Hemorrhagic activity of culture supernatants (A), its graphic representation (B) and inhibition with chemical inhibitors (C). 100 μl of secreted proteins (20 to 100 μg) were subcutaneously (sc) injected into the femoral artery region for observation of hemorrhagic changes. The fur over the femoral artery region was removed to allow observation of a 1.5 to 2.5 cm2 area of skin. Control mice were administered equal amount of phosphate-buffered saline. Error bars indicate standard deviations.