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Figure 2 | BMC Infectious Diseases

Figure 2

From: Infection of cells expressing CXCR4 mutants lacking N-glycosylation at the N-terminal extracellular domain is enhanced for R5X4-dualtropic human immunodeficiency virus type-1

Figure 2

CXCR4 cell surface expression. The CXCR4 coding sequence was mutated in the pcDNA3.1 plasmid, cloned into pBABE-puro and finally expressed in GHOST.CD4 cells. Cell surface expression was monitored by flow cytometry using phycoerythrin labeled anti-CXCR4 mAb 12G5. Coreceptor expression is expressed as % of CXCR4 wt expression of GHOST.CD4.CXCR4 cells. Error bars are calculated based on three independent experiments.

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