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Nasopharyngeal microbiota evaluation in three cohorts of children in the Romanian pediatric population
BMC Infectious Diseasesvolume 14, Article number: O21 (2014)
We performed a screening study for nasopharyngeal carriage of Staphylococcus spp. in immunocompetent children aged 7-10 years old, attending a community school in central Bucharest (group 1), and in two groups of immunosuppressed children: children with hemato-oncologic diseases (lymphoma/leukemia) admitted to the Fundeni Clinical Institute, Bucharest (ages 2-10 years, group 2), and institutionalized children with vertically transmitted HIV infection, from the National Institute for Infectious Diseases “Prof. Dr. Matei Balş”, Bucharest (ages 1-10 years, group 3).
We analyzed data from 139 pharyngeal swabs (35.3% in group 1, 56.1% group 2 and 8.6% group 3), and 143 nasal swabs (37.1% group 1, 54.5% group 2 and 8.4% group 3). Pharyngeal cultures were positive for Staphylococcus spp. in 28.6% of children in group 1, 11.5% in group 2 (p=0.00755 vs. group 1) and 16.7% in group 3 (p=0.20045 vs. group 1). Of the positive pharyngeal samples, 92.9% were S. aureus in group 1, 100% in group 2 and 100% in group 3.
Nasal cultures were positive for Staphylococcus spp. in 84.9% of children in group 1, 48.7% group 2 (p=0 vs. group 1) and 50.0% group 3 (p=0.00391 vs. group 1). Of the positive nasal samples, 62.2% were identified as S. aureus in group 1, 94.6% in group 2 (p=0.00027 vs. group 1) and 66.7% in group 3.
Pharyngeal carriage of Staphylococcus strains was low, however when positive, most strains were S. aureus. Nasopharyngeal carriage was significantly higher in immunocompetent children from the community compared to immunodepressed children. When present, S. aureus had a higher prevalence compared to coagulase-negative staphylococci (CoNS), particularly in immunodepressed children.
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2) Carol Davila University of Medicine and Pharmacy, Young Researchers Projects Grant Competition, contract number 28.335/04.11.2013.