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  • Open Access

Prevalence and co-infection study of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis among symptomatic women using PCR assay

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  • 1,
  • 1,
  • 2,
  • 2,
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  • 1Email author
BMC Infectious Diseases201414 (Suppl 3) :P5

  • Published:


  • Infectious Disease
  • Infertility
  • Acute Illness
  • Chlamydia Trachomatis
  • Neisseria Gonorrhoeae


Sexually transmitted infections (STIs) are one of the major causes of acute illness, infertility, long term disability and death for millions of men, women and infants globally. Trichomonas vaginalis, Neisseria gonorrhea and Chlamydia trachomatis are well established agents of STIs leading to vaginal discharge in women. However, the prevalence and co infection patterns among symptomatic women is lacking in India. The present study aims to determine the prevalence and co infection of Chlamydia trachomatis, Neisseria gonorrhoeae and Trichomonas vaginalis, in women with vaginal discharge attending OPD, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi.


Dry swab samples were obtained and DNA was extracted used as template for PCR amplification using primers targeting pfoB, gyr A and orf1 gene for diagnosis of T. vaginalis, C. trachomatis and N. gonorrhoeae respectively to estimate prevalence of co-infection.


A total of 335 women were studied (mean age 23.2 years), out of which 22 women (6.56 %) women were infected with had at least one pathogen. One woman (4.54 %) was co–infected with all three pathogens. Amongst 335 women, 18 women (5.37 %) tested positive for T. vaginalis, 02 women (0.59 %) tested positive for C. trachomatis and 1 woman (0.29 %) tested for N. gonorrhea by all the PCR assays, whereas 313 women (93.43 %) tested negative for the all three pathogens.


The results demonstrated that it is necessary to test for all three pathogens namely T. vaginalis, N. gonorrhea and C. trachomatis in women with vaginal discharge.

Authors’ Affiliations

Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi, 110007, India
Department of Obstetrics & Gynecology Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, 110029, India


© Sonkar et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.