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  • Poster presentation
  • Open Access

Spectrum and prognostic significance of parasitic infection in ART naïve HIV patients

BMC Infectious Diseases201414 (Suppl 3) :P28

  • Published:


  • Polyvinyl Alcohol
  • Parasitic Infection
  • Stool Sample
  • Intestinal Parasite
  • Ether Concentration


It is a well-known fact that HIV/AIDS patients are vulnerable to many diseases which result in serve morbidity and mortality. The purpose of the study is to assess the prevalence and pattern of intestinal parasites in ART naïve patients.


Field sampling started from July 2012 to July 2013 which included a total of 163 HIV seropositive patients. After taking an informed consent and recorded CD4 date, stool samples were collected for screening of parasites. Two stool samples were collected in two vials, one with formalin and other with polyvinyl alcohol (PVA). Saline wet mount and Lugols Iodine mount was done by direct microscopy and a formalin ether concentration method to detect ova, trophozoites, cysts, and larvae of intestinal parasites.


A total of 57 (34.9%) patients were found positive from 163 newly registered HIV patients. The prevalence of parasitic infection was more in the age group of 31-40 years in males, where as in females 21-30 years. The most common isolate was Hook worm (29, 50.8%) followed by Isospora (13, 22%) Cryptosporidium (9, 15.7%), E. histolytica (5, 8.7%), and Giardia intestinalis (1, 1.75%). Most of the cases were asymptomatic and the CD4 count was ≤ 350 mm3. Coccidian parasites always presented diarrhea and had a CD4 count of <200 mm3. In this study the most presenting symptoms were constipation followed by weight loss and anemia.


This high spectrum of infection before ART concludes that early screening should be made mandatory and lower CD4 counts mostly associated with presence of coccidian parasites with diarrhea as presenting symptom.

Authors’ Affiliations

Department of Microbiology, Rural Medical College, Loni, Maharashtra, India


© Padmjakshi and Saini; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.