- Poster presentation
- Open Access
Complete genome sequence of new unique recombinant HIV type 1 isolated from a child born to an HIV-infected mother
© Sasinovich et al; licensee BioMed Central Ltd. 2014
- Published: 23 May 2014
- Public Health
- Internal Medicine
- Infectious Disease
- Phylogenetic Analysis
- Genome Sequence
HIV-1 RNA was extracted from plasma, reverse transcribed, and amplified using sets of primers designed to span the complete genome of HIV-1. Contig assembled in SeqMan®, DNAStar. Recombination analysis was performed using REGA HIV Subtyping Tool. Phylogenetic analysis was made in MEGA4 software with Kimura-2-parametres.
Analysis of complete genome shows that recombinant Mos is a new HIV-1 URF with gag/pol mosaic structure composed of parental subtypes A and B and rest genome composed of subtype A. Phylogenetical analysis had shown that HIV-1 subtype A ancestor is AY500393 Russia, subtype B - DQ207943 Georgia.
This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from Russia and Georgia.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.