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Genetic susceptibility to Chagas disease cardiomyopathy: involvement of several genes of the innate immunity and chemokine-dependent migration pathways
BMC Infectious Diseasesvolume 13, Article number: 587 (2013)
Chagas disease, caused by the protozoan Trypanosoma cruzi is endemic in Latin America. Thirty percent of infected individuals develop chronic Chagas cardiomyopathy (CCC), an inflammatory dilated cardiomyopathy that is, by far, the most important clinical consequence of T. cruzi infection. The others remain asymptomatic (ASY). A possible genetic component to disease progression was suggested by familial aggregation of cases and the association of markers of innate and adaptive immunity genes with CCC development. Migration of Th1-type T cells play a major role in myocardial damage.
Our genetic analysis focused on CCR5, CCL2 and MAL/TIRAP genes. We used the Tag SNPs based approach, defined to catch all the genetic information from each gene. The study was conducted on a large Brazilian population including 315 CCC cases and 118 ASY subjects.
The CCL2rs2530797A/A and TIRAPrs8177376A/A were associated to an increase susceptibility whereas the CCR5rs3176763C/C genotype is associated to protection to CCC. These associations were confirmed when we restricted the analysis to severe CCC, characterized by a left ventricular ejection fraction under 40%.
Our data show that polymorphisms affecting key molecules involved in several immune parameters (innate immunity signal transduction and T cell/monocyte migration) play a role in genetic susceptibility to CCC development. This also points out to the multigenic character of CCC, each polymorphism imparting a small contribution. The identification of genetic markers for CCC will provide information for pathogenesis as well as therapeutic targets.
Chagas disease (American trypanosomiasis) is caused by the protozoan Trypanosoma cruzi and transmitted by the reduviid bug. It occurs exclusively in the Americas, particularly in poor, rural areas of Mexico, Central America, and South America. The disease remains endemic in Latine America where the vector-based transmission is still active in some countries. Imported disease is increasingly recognized as an emerging problem in the USA and Europe due to immigration from Latin America. It is estimated that as many as 8–9 million people have Chagas disease. Approximately, 40 million people are currently at risk of infection . Decades after acute infection, approximately 30% of infected individuals develop Chronic Chagas cardiomyopathy (CCC), one of the most important consequence of T. cruzi infection. CCC is an inflammatory dilated cardiomyopathy, with a potentially fatal outcome. 5 to 10% of infected individuals develop digestive disease. The remaining two-thirds of infected individuals remain asymptomatic (ASY) and free from heart disorders for life . 20,000 deaths attributable to Chagas disease occur annually, typically due to CCC . Heart failure due to CCC has a worse prognosis with 50% shorter survival when compared to other cardiomyopathies of different etiologies [4, 5].
The dynamics of the immune response to T. cruzi is that of a persistent infection with an obligatory intracellular parasite. During acute T. cruzi infection, T. cruzi pathogen-associated molecular patterns (PAMPs) trigger innate immunity in multiple cell types , which release proinflammatory cytokines and chemokines, such as IL-1, IL-6, IL-12, IL-18, TNF-α, CCL2, CCL5, and CXCL9 activating and mobilizing migration of cascades of inflammatory cells [7, 8]. Antigen-presenting cells subsequently elicit a strong T cell and antibody response against T. cruzi, where IL-12 and IL-18 drive the differentiation of IFN-γ-producing T. cruzi–specific Th1 T cells which migrate to sites of T. cruzi-induced inflammation, including the myocardium, in response to locally produced chemokines [9, 10]. Th1 T cell and antibody responses lead to control but not complete elimination of tissue and blood parasitism, establishing a low-grade chronic persistent infection by T. cruzi. As a result of persistent infection, both CCC and ASY chronic Chagas disease patients show a skewed Th1-type immune response [11, 12], but those who develop Chagas cardiomyopathy display a particularly strong Th1-type immune response with increased numbers of IFN-γ-producing T cells in peripheral blood mononuclear cells (PBMC)  as well as plasma TNF-α in comparison with uninfected or ASY patients . PBMC of CCC patients also display increased levels of IFN-γ- or TNF-α producing CCR5/CXCR3+ CD4+ T cells [15, 16]. In addition, CCC patients display a reduced number of CD4+CD25highIL-10+ and CD4+CD25highFoxP3+ regulatory T cells in their peripheral blood as compared to patients in the ASY form of Chagas disease, suggesting such cells may play a role in the control of the intensity of inflammation in chronic Chagas disease [15, 17]. Furthermore, PBMC from CCC patients displayed increased numbers of CD4+CD25highFoxP3+CTLA-4+ T cells, and decreased numbers of as compared to ASY patients. These reports suggest that a smaller CD4+FoxP3+/CD25+ Treg compartment with deficient suppressive activity exists in CCC patients, leading to uncontrolled production of Th1 cytokines . Circulating CD4+IL-17+ T cells appear in low frequency in PBMC from CCC patients as compared with ASY patients and non-infected individuals [18, 19]. On the whole, these results suggest that proinflammatory cells and cytokines are markers associated with progression to CCC, whereas the production of IL-10, IL-17 and increased numbers of regulatory T cells are markers of protection from CCC development, indicating that failure to regulate Th1 responses may be the underlying immune defect of patients who progress to CCC.
The exacerbated Th1 response observed in the PBMC of CCC patients is reflected on the Th1-rich myocardial inflammatory infiltrate, with mononuclear cells predominantly producing IFN-γ and TNF-α, with lower production of IL-4, IL-6, IL-7, and IL-15 [7, 20, 21]. It has recently been shown that CCL5+, CCXCL9+, CCR5+, CXCR3+ cells were abundant in CCC myocardium, and mRNA levels of the Th1-chemoattracting chemokines CXCL9, CXCL10, CCL2 (also known as MCP-1), CCL3, CCL4, CCL5; along with CCL17, CCL19, CCL21 and their receptors were also found to be upregulated in CCC heart tissue [12, 22]. Importantly, median expression of CCL5, a CCR5 ligand, was the highest among all chemokines tested (166-fold increase over control). Significantly, the intensity of the myocardial infiltrate was positively correlated with CXCL9 mRNA expression. Moreover, a single nucleotide polymorphism in the CXCL9 gene, associated with a reduced risk of developing severe CCC in a cohort study, was associated with reduced CXCL9 expression and intensity of myocarditis in CCC . These results are consistent with a major role of locally produced Th1-chemoattractant chemokines in the accumulation of CXCR3/CCR5+ Th1 T cells in CCC heart tissue .
Familial aggregation of CCC has been described, suggesting that there might be a genetic component to disease susceptibility . Several genes were associated to an increased risk to develop cardiomyopathy (HLA, MHC, TNF, IL1A, IL1B, IL1RN, IL10, IL12B, TIRAP, CCL2, BAT1, LTA, IKBL, CCR5, MIF, IFNG, CXCL9, CXCL10) [25–50]. So far, up to 30 case control studies were done (see for review [51–53]). These studies often led to inconclusive results that may be explained in different ways: a) the use of seronegative subjects as controls which are inadequate controls, since it is unknown whether they were exposed to the pathogen; b) the relatively small size of the study groups which affected the power (the probability) to detect an association; c) the number of tested SNPs; d) the highly heterogeneous genetic background of the study population due to admixture; e) the sex ratio known to exist has not been taken in consideration .
Among these susceptibility studies, putative implication of genes crucially involved in the innate immunity-such as the Toll like receptors (TLR) and some of its most relevant signalling molecules like TIRAP was searched for. Two studies on the TLR and TIRAP failed to identify disease associations with TLR 1,2, 5, 6 and 9; in one of the reports an association was found with a TLR4 SNP among Chilean chagasic patients , while in the second study – which enrolled nearly double the number of Brazilian Chagasic individuals - no association was found with TLR4, but instead with TIRAP S180L heterozygosity . Chemokines are key players in controlling migration of specific cell types bearing their receptors to sites of tissue inflammation, and associations between CCR5 –involved in T cell and macrophage migration and CCL2 –involved in monocyte migration - with CCC were reported [42, 47, 48]. Both processes, TLR signaling and chemokine-mediated cell migration are of paramount importance in Chagas disease and are key to the pathogenesis of CCC. Here, we conducted a study focusing on TIRAP, CCL2 and CCL5. Thorough genetic analysis, testing multiple tag SNPs per gene and thus detecting any possible relevant genetic variants in a large Brazilian population and ASY subjects as controls we could have a sensitive assessment of the contribution of genetic variants in prognosis to CCC either confirming or finding additional associated SNPs in the mentioned genes. This can be considered a candidate gene replication study, performed with a larger cohort of Chagas patients and only comparing CCC to the asymptomatic seropositive (ASY) patient group. Significant associations were found for CCR5, CCL2, and TIRAP genes.
Written informed consent was obtained from all the patients, in accordance with the guidelines of the various internal review boards of all the involved institutions. The protocol was also approved by the INSERM Internal Review Board and the Brazilian National Ethics in Research Commission (CONEP). All the patients enrolled in this study were over 21 years old so paternal consent was not required. In the case of samples from heart donors, written informed consent was obtained from their families. Investigations were conformed to the principles outlined in the declaration of Helsinki.
The diagnostic criteria for Chagas disease included the detection of antibodies against T. cruzi in at least two of three independent serological tests (EIA [Hemobio Chagas; Embrabio São Paulo], indirect immunofluorescence assays [IFA-immunocruzi; Biolab Merieux], and indirect hemagglutination tests [Biolab Merieux]) . All Chagas disease patients underwent standard electrocardiography and echocardiography. Echocardiography was performed at the hospital, with a Sequoia model 512 echocardiograph with a broad-band transducer. Left ventricular dimensions and regional and global function, including the recording of left ventricular ejection fraction (LVEF), were evaluated with a two-dimensional, M-mode approach, in accordance with the recommendations of the American Society of Echocardiography. ASY subjects had no electrocardiography and echocardiography changes. CCC patients presented typical conduction abnormalities (right bundle branch block and/or left anterior division hemiblock) . CCC patients with significant left ventricular systolic dysfunction (LVEF <40%) were classified as having severe CCC, whereas those with no significant ventricular dysfunction (LVEF ≥40%) were classified as having moderate CCC. We selected 40% as arbitrary cutoff value that has been previously used to define significant ventricular dysfunction by our group and others [22, 57, 58].
Study population for polymorphism analysis
The patients and ASY controls were born and raised in rural areas of Sao Paulo, Minas Gerais and Bahia states and enrolled in one of the study centers (Incor, FMUSP, FMRP, UFTM, IDPC). Patients with digestive forms were excluded of this study. Patients were classified as ASY (n = 118) or as having CCC (n = 315). ASY individuals were used as the control subjects for this study because they were from the same areas of endemicity as the patients with CCC, had encountered the parasite and had tested seropositive for T. cruzi infection, but the infection had not progressed to CCC. Of the 118 ASY subjects, 45.3% were male, whereas in the CCC patients group, this percentage reaches 61.3%. The difference in sex distribution between the groups was significant (p = 1.21E-4; OR = 2.126; 95% CI: 1.450 – 3.12). It is well known that male patients infected with T. cruzi have a higher risk of progression to CCC than female patients [54, 59, 60]. Of 315 patients with CCC, 106 (42 men [39.6%] and 64 women [60.4%]) showed no significant ventricular dysfunction and were thus classified as having moderate CCC, whereas 199 (144 men [72.4%] and 55 women [27.6%]) had severe ventricular dysfunction and were classified as having severe CCC. Data for left ventricular ejection fraction were missing for 10 patients with CCC. So, when we compared moderate patients to severe patients, these 10 individuals were excluded from the analysis. Regarding progression of the ASY cases to CCC, the yearly progression rate –regardless of age group- is ca. 1-2%/year. The average age of Subjects with asymptomatic form was above 55 years. Taking into account that they were all born in endemic areas before vector transmission was interrupted, it is likely that in most if not all cases vector-borne infection occurred in early childhood. The odds that a significant number of such mature patients convert to CCC, and that this thwarts our statistical calculation is rather low; however, this is a pitfall of all cross sectional studies on diseases that display progression.
Blood samples and DNA preparation
For each subject, 5 to 15 ml of blood were collected in EDTA tubes. Genomic DNA was isolated on a silica-membrane according to the manufacturer’s protocol (QIAamp DNA Blood Max Kit, Qiagen, Hilden, Germany).
Tag single nucleotide polymorphisms (SNPs) were selected on the basis of HapMap Data for the Caucasian and Yoruba reference populations. Tag SNPs were selected within a region extending 5 kb on either side of the candidate gene. The minor allele frequency (MAF) cut off value was arbitrarily set at 20% (so the markers characterized by a MAF < 20% were excluded from the analysis by lack of power). In each reference population, the markers with MAF > 20% are included in different blocks of correlation (based on the r2 values). One marker in each block was selected and considered as a Tag SNPs. Indeed, markers located in the same block of correlation gave the same genetic information in association studies. Tag SNPs characterised by a MAF over 20% on at least one reference population were selected. These Tag SNPs were defined to catch all the genetic information from the candidate gene. We selected three tag SNPs for CCR5, six tag SNPs for CCL2 and six tag SNPs for MAL/TIRAP genes. Taking into account a disease with a prevalence of 30%, a cutoff for significant association of 0.05, for a genotype relative risk of 1.3, the probability to detect a real association reaches 63% with 315 chronic cases and 118 ASY controls. We decided to use a cut off of 20% instead of 10% or 15%. For lower cut off, the number of Tag SNPs will increase and it will request a seriously large your study population to have a good statistical power.
Most of the genotyping was done with the Golden Gate genotyping assay (Illumina, San Diego, USA). In some cases, genotyping assays were performed with the TaqMan system (Applied Biosystems, Foster City, USA) according to the manufacturer’s instructions.
SPSS Statistics software v. 17.0 (IBM, Armonk, USA) was used for statistical analyses. We performed stepwise binary logistic regression analysis on the whole population, to analyse the relationship between the probability of an individual to develop chronic Chagas cardiomyopathy and the main covariates (sex and polymorphisms). Sex was considered as a binary covariate. In our stepwise binary logistic regression analysis, genotypes were considered as binary covariates. Indeed, for each polymorphism we had two alleles (A frequent one; a rare one). So, we obtained three genotypes (AA, Aa and aa). In our stepwise binary logistic regression analysis, genotypes were considered as binary covariates. So, we performed three different analyses (Analysis 1: AA vs Aa + aa (we supposed that the a allele is dominant); Analysis 2: AA + aa vs Aa (we supposed that the heterozygote carriers are different from the homozygote ones); Analysis 3: AA + Aa vs aa (we supposed that the A allele is dominant)). The best results are indicated in Tables 1, 2 and 3.
In multivariates analyses, several polymorphisms and gender were included as covariates. All the covariates are analyzed in the same time. In a stepwise approach, the worse associated covariate (non significant) is removed and the analysis is run again up to keep only significant associated covariates.
Results and discussion
Fifteen Tag SNPs were genotyped successfully on our original cohort including ASY subjects (n = 118) and CCC patients (n = 315) (Table 4). The genotyping steps were done successfully for all the Tag SNPs. The genotype distribution of each SNP is summarized in Table 5. All the SNPs were in Hardy-Weinberg equilibrium on the ASY individuals considered as control subjects (p > 0,001) (Table 6).
Polymorphisms rs3176763C/A and rs11575815A/T, around the CCR5 gene, are associated to an increased risk of CCC
Three tag SNPs were genotyped for the CCR5 gene. In the CCC subjects group, 266 (84.4%) subjects carried the rs3176763C/C genotype whereas 110 (94.0%) of the ASY controls carried this genotype. This difference was significant in an univariate analysis including also the gender as covariate (p = 0.006; OR = 1.79; 95% CI: 1.18-2.70) (see Table 1).
For the rs11575815A/T polymorphism, 278 (90.3%) CCC subjects carried the genotypes rs11575815A/A or rs11575815A/T versus 93 (82.3%) for the ASY controls. This difference was significant (p = 0.030; OR = 1.41; 95% CI: 1.03-1.92) (see Table 1).
We performed a multivariate analysis (binary regression, stepwise procedure) to confirm the associations found previously in univariate analysis. Similarly to the univariate analysis, the genotypes were considered as binary variables. In this analysis, we included rs3176763C/A, rs11575815A/T and the gender as covariates. Polymorphism rs3176763C/A (p = 0.014; OR = 1.69; 95% CI: 1.11-2.57) and the gender (p = 0.002; OR = 2.04; 95% CI: 1.31-3.19) were still significantly associated to CCC (see Table 7). A trend of association was detected for rs11575815A/T (p = 0.077; OR = 1.33; 95% CI: 0.97-1.82).
When we compared the ASY subjects to severe CCC patients (left ventricular ejection fraction value under 0.4%), only the association of rs3176763C/A was maintained in univariate analysis (p = 0.005; OR = 1.88; 95% CI: 1.20-2.94) (see Table 2). These two markers (rs3176763C/A and rs11575815A/T) did not discriminate moderate CCC from severe CCC (p > 0.5).
Polymorphisms rs4586T/C, rs3917891C/T and rs2530797A/G, around the CCL2 gene, are associated to an increased risk of CCC
Six tag SNPs were genotyped for the CCL2 gene. In the CCC subjects group, 74 (24.0%) carried the rs4586T/T genotype whereas 38 (34.5%) of the ASY controls carried this genotype. This difference was significant in an univariate analysis (p = 0.032; OR = 1.30; 95% CI: 1.02-1.65) (see Table 1).
For the rs3917891C/T polymorphism, 264 (86.0%) CCC subjects carried the rs3917891C/C genotype versus 107 (93.9%) for the ASY controls. This difference was significant (p = 0.037; OR = 1.56; 95% CI: 1.03-2.37) (see Table 1).
For the rs2530797A/G polymorphism, 163 (52.9%) CCC subjects carried the rs2530797A/A genotype versus 47 (41.6%) for the ASY controls. This difference was significant (p = 0.028; OR = 1.28; 95% CI: 1.03-1.60) (see Table 1).
The same polymorphisms were associated when we compared the ASY subjects to severe CCC patients (rs4586T/C: p = 0.034; OR = 1.34; 95% CI: 1.02-1.75; rs3917891C/T: p = 0.053; OR = 1.55; 95% CI: 1.00-2.41; rs2530797A/G: p = 0.005; OR = 1.42; 95% CI: 1.11-1.82) (see Table 2).
We performed multivariate analysis including these three polymorphisms and the gender as covariates. When we compared the ASY subjects to CCC patients, only the polymorphism rs2530797A/G and the gender remained significantly associated (rs2530797A/G: p = 0.022; OR = 1.30; 95% CI: 1.04-1.64; gender: p = 0.001; OR = 2.09; 95% CI: 1.33-3.28) (see Table 7).
The same result was obtained, when we compared the ASY subjects to severe CCC patients (rs2530797A/G: p = 8.51×10-7; OR = 1.46; 95% CI: 1.13-1.88; gender: p = 0.004; OR = 3.59; 95% CI: 2.16-5.97). These three markers (rs4586T/C, rs3917891C/T and rs2530797A/G) did not discriminate moderate CCC from severe CCC (p > 0.16).
Polymorphism rs8177376A/C, around the MAL/TIRAP gene, is associated to an an increased risk of CCC
Six tag SNPs were genotyped for the MALTIRAP gene. For the rs8177376A/C polymorphism, 230 (75.4%) CCC subjects carried the rs8177376A/A genotype versus 63 (54.9%) for the ASY controls. This difference was significant (p = 0.004; OR = 1.42; 95% CI: 1.12-1.80) (see Table 1). The same result was obtained when the analysis was restricted to severe CCC (p = 0.005; OR = 1.46; 95% CI: 1.12-1.91) (see Table 2).
A statistically significant difference was also detected for the rs17866704T/C polymorphism (p = 0.023; OR = 1.31; 95% CI: 1.04-1.66) (see Table 1). In our cohort, 175 ((57.4%) CCC subjects carried the rs17866704T/T genotype versus 80 (70.8%) for the ASY controls. The two SNPs remained associated in a multivariate analysis (see Table 7).
Some trend of association was detected for the rs17866704T/C polymorphism when we compared the ASY subjects to the severe CCC patients (p = 0.087; OR = 1.25; 95% CI: 0.97-1.62) (see Table 2). The rs8177376A/C marker did not discriminate moderate CCC from severe CCC (p > 0.57).
The associations of the CCL2 and MAL/TIRAP genes were confirmed in a cohort from the original reports
The original data reporting association between the CCL2 and TIRAP genes were done by Ramasawmy et al.[41, 42]. These studies were done on 169 patients with CCC and 76 T. cruzi infected ASY individuals. Our present study population is partially overlapping with the original one described by Ramasawmy et al. So, we repeated the analysis for these two genes on our cohort after removing the common subjects. This independent cohort includes 110/118 ASY subjects and 281/315 CCC patients. Of 281 patients with CCC, 192 had severe ventricular dysfunction and were classified as having severe CCC. The genotype distribution of the CCL2 and TIRAP Tag SNPs, on this independent cohort, is summarized in Table 8. In association studies, the gender was also included as covariates.
For the CCL2rs2530797A/G polymorphism, 132 (50.6%) CCC subjects carried the rs2530797A/A genotype versus 25 (33.8%) for the ASY controls (see Table 8). This difference was significant (p = 0.007; OR = 1.4 p = 0.007; OR = 1.46; 95% CI: 1.11-1.926) (see Table 8). The same polymorphism remained associated when we compared the ASY subjects to severe CCC patients (p = 0.002; OR = 1.59; 95% CI: 1.19-2.13) (see Table 3).
For the MAL/TIRAPrs8177376A/C polymorphism, 195 (75.6%) CCC subjects carried the rs8177376A/A genotype versus 42 (61.8%) for the ASY controls (see Table 8). This difference was significant on the whole independent cohort (p = 0.037; OR = 1.36; 95% CI: 1.19-1.80) (see Table 3). The same result was obtained when the analysis was restricted to severe CCC (p = 0.046; OR = 1.36; 95% CI: 1.05-1.85) (see Table 3).
A trend of association was detected for the rs17866704T/C polymorphism in both analyses (p = 0.051; OR = 1.32; 95% CI: 1.00-1.76) (see Table 3) and (p = 0.095; OR = 1.29; 95% CI: 0.96-1.74) (see Table 3).
In order to detect interaction between the candidate genes a multivariate stepwise binary logistic regression analysis was performed on ASY subjects and CCC patients (see Table 9). In this analysis, we included the gender, rs11575815A/T, rs2530797A/G, rs8177376A/C and rs17866704T/C as covariates. Polymorphisms CCR5rs3176763C/A (p = 0.007; OR = 1.879; 95% CI: 1.19-1.89), TIRAP rs8177376A/C (p = 0.007; OR = 1.393; 95% CI: 1.09-1.77) and the gender (p = 0.001; OR = 2.226; 95% CI: 1.39-3.55) were still significantly associated to CCC (see Table 9). However, if we want to add a significant number of genes and polymorphisms at the first step of the multivariate analysis, the study population (which is one of the largest described so far) is underpowered. So, we are working toward obtaining a cohort between 1,500 and 2,000 subjects that would enable us to assess whether possessing a given combination of alleles in several SNPs contribute more strongly for prognosis than the individual SNPs.
We conducted an association study on several previously studied candidate genes on a Brazilian population. Whereas previously studies were done on a limited number of subjects (CCC patients ranges from 27 to 169, ASY controls ranges from 27 to 132) our study was done on a main cohort including 433 Chagas disease patients from the states of Sao Paulo, Minas Gerais and Bahia states. These patients were classified as seropositive ASY (n = 118) or as having CCC (n = 315). Whereas, previous studies were done on a limited number of SNPs, here, a Tag SNPs approach was applied to catch all the genetic information from each candidate gene.
For the CCR5 gene, two markers were associated to CCC (rs3176763C/A and rs11575815A/T). The association of rs3176763C/A was confirmed in a multivariate analysis or in a univariate analysis focusing only on severe CCC cases. rs3176763C/A polymorphism is located in the promoter of the gene and may affect the binding of transcription factors. Although these SNPs were not studied before, is in line with the literature in studies performed in other Latin American countries with diverse ethnic compositions, where several SNPs were located in the 5′UTR of the CCR5 gene where they may influence binding of regulatory elements to gene expression control regions [22, 47, 48, 61]. As suggested by Florez et al., these polymorphisms do not act independently . Multiple polymorphic changes in the promoter may influence in a differential way the levels of CCR5 expression and the type of cell in which it is expressed. So, it’s more appropriate to talk about a susceptibility haplotype rather than individual SNPs. The content and the length of this haplotype may vary from one population to the other. The subsets of patients that develop Chagas cardiomyopathy display an exacerbated Th1 immune response. The relevance of the CCR5 and CXCR3 chemokine–chemokine receptor axis in Th1 cell migration to the heart has been demonstrated in experimental models [62–64] and in CCC .
For the CCL2 gene, three markers were associated with CCC (rs4586T/C, rs3917891C/T and rs2530797A/G). Only the rs2530797A/G polymorphism remains associated into multivariate analysis. The rs4586C/T polymorphism is a synonymous marker, whereas the two other SNPs are located into the 3′ region of the gene and may affect stability of the transcript or the binding of regulatory elements. The previous associated marker reported by Ramasawmy et al. is located into the promoter region (CCL2-2518A-G known as rs1024611) . These results are absolutely not in discrepancy. Indeed, our tag SNPs were selected on the CEU and YRI reference populations. In these two reference populations the rs2530797A/G and rs1024611 are in strong linkage disequilibrium (previous associated marker) (D’ = 1). So the genetic involvement of the CCL2 gene in the control of the human susceptibility to chronic disease is confirmed. Patients with severe Chagas disease had elevated plasma concentrations of TNF-α and CCL2. Moreover, there is a good correlation between levels of these proteins (especially TNF-α) and the degree of left ventricular dysfunction . Real-time quantitative PCR analysis in human CCC myocardium showed that the gene expression levels of CCL2 was selectively upregulated , reinforcing the importance of regulation of CCL2 expression in the pathogenesis of CCC.
For the TIRAP gene, only one marker, located into the 3′UTR region of the gene, was strongly associated (rs8177376A/C) and may affect stability of the transcript or the binding of regulatory elements. This result is in line with previous association reported by Ramasawmy et al. who reported a non-synonimous polymorphism at a coding region (TIRAP975C/T, S180L known also as rs8177374). Indeed these two SNPs (rs8177376 and rs8177374) are in strong linkage disequilibrium. This gene encodes for a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which promote cytokine secretion and the inflammatory response.
Our data show beyond reasonable doubt that polymorphisms affecting key molecules involved in several immune parameters (innate immunity signal transduction and T cell/monocyte migration to inflammatory regions) play a role in genetic susceptibility to CCC development. However, the functional impact of these markers remains unknown. This also points out to the multigenic character of CCC, each polymorphism imparting a small contribution.
When all the genetic markers will be identified, we will be able to performed multivariate analyses using several genes (gene polymorphisms) as covariates. In order to perform this kind of analysis it is essential to enroll a study population including at least 1,500 and 2,000 cases and 1000 ASY controls. It will allow us to detect gene–gene interactions and additive or antagonist effects between the associated polymorphisms. A panel of markers will be defined to early detect individuals with a highest risk to develop chronic Chagas cardiomyopathy. It will provide information for pathogenesis as well as therapeutic targets. The identification of these marker sets may also have a combined prognostic value for disease progression at the individual patient level, allowing close follow up and early treatment of those carrying high-risk genetic signatures.
Chronic Chagas cardiomyopathy
T helper 1
Single nucleotide polymorphism
- T. cruzi:
Pathogen-associated molecular patterns
Tumor necrosis factor
Left ventricular ejection fraction.
Schofield CJ, Jannin J, Salvatella R: The future of Chagas disease control. Trends Parasitol. 2006, 22 (12): 583-588. 10.1016/j.pt.2006.09.011.
Coura JR: Chagas disease: what is known and what is needed–a background article. Mem Inst Oswaldo Cruz. 2007, 102 (Suppl 1): 113-122.
Kirchhoff LV, Weiss LM, Wittner M, Tanowitz HB: Parasitic diseases of the heart. Front Biosci. 2004, 9: 706-723. 10.2741/1255.
Barbosa AP, Cardinalli Neto A, Otaviano AP, Rocha BF, Bestetti RB: Comparison of outcome between Chagas cardiomyopathy and idiopathic dilated cardiomyopathy. Arq Bras Cardiol. 2011, 97 (6): 517-525. 10.1590/S0066-782X2011005000112.
Bestetti RB, Muccillo G: Clinical course of Chagas’ heart disease: a comparison with dilated cardiomyopathy. Int J Cardiol. 1997, 60 (2): 187-193. 10.1016/S0167-5273(97)00083-1.
Bafica A, Santiago HC, Goldszmid R, Ropert C, Gazzinelli RT, Sher A: Cutting edge: TLR9 and TLR2 signaling together account for MyD88-dependent control of parasitemia in Trypanosoma cruzi infection. J Immunol. 2006, 177 (6): 3515-3519.
Reis MM, Higuchi Mde L, Benvenuti LA, Aiello VD, Gutierrez PS, Bellotti G, Pileggi F: An in situ quantitative immunohistochemical study of cytokines and IL-2R + in chronic human chagasic myocarditis: correlation with the presence of myocardial Trypanosoma cruzi antigens. Clin Immunol Immunopathol. 1997, 83 (2): 165-172. 10.1006/clin.1997.4335.
Bilate AM, Cunha-Neto E: Chagas disease cardiomyopathy: current concepts of an old disease. Rev Inst Med Trop Sao Paulo. 2008, 50 (2): 67-74. 10.1590/S0036-46652008007500001.
Muller U, Kohler G, Mossmann H, Schaub GA, Alber G, Di Santo JP, Brombacher F, Holscher C: IL-12-independent IFN-gamma production by T cells in experimental Chagas’ disease is mediated by IL-18. J Immunol. 2001, 167 (6): 3346-3353.
Rocha Rodrigues DB, dos Reis MA, Romano A, Pereira SA, Teixeira Vde P, Tostes S, Rodrigues V: In situ expression of regulatory cytokines by heart inflammatory cells in Chagas’ disease patients with heart failure. Clin Dev Immunol. 2013, 2012: 361730-
Ribeirao M, Pereira-Chioccola VL, Renia L, Augusto Fragata Filho A, Schenkman S, Rodrigues MM: Chagasic patients develop a type 1 immune response to Trypanosoma cruzi trans-sialidase. Parasite Immunol. 2000, 22 (1): 49-53. 10.1046/j.1365-3024.2000.00260.x.
Cunha-Neto E, Dzau VJ, Allen PD, Stamatiou D, Benvenutti L, Higuchi ML, Koyama NS, Silva JS, Kalil J, Liew CC: Cardiac gene expression profiling provides evidence for cytokinopathy as a molecular mechanism in Chagas’ disease cardiomyopathy. Am J Pathol. 2005, 167 (2): 305-313. 10.1016/S0002-9440(10)62976-8.
Cunha-Neto E, Kalil J: Heart-infiltrating and peripheral T cells in the pathogenesis of human Chagas’ disease cardiomyopathy. Autoimmunity. 2001, 34 (3): 187-192. 10.3109/08916930109007383.
Talvani A, Rocha MO, Barcelos LS, Gomes YM, Ribeiro AL, Teixeira MM: Elevated concentrations of CCL2 and tumor necrosis factor-alpha in chagasic cardiomyopathy. Clin Infect Dis. 2004, 38 (7): 943-950. 10.1086/381892.
Gomes JA, Bahia-Oliveira LM, Rocha MO, Martins-Filho OA, Gazzinelli G, Correa-Oliveira R: Evidence that development of severe cardiomyopathy in human Chagas’ disease is due to a Th1-specific immune response. Infect Immun. 2003, 71 (3): 1185-1193. 10.1128/IAI.71.3.1185-1193.2003.
Talvani A, Rocha MO, Ribeiro AL, Correa-Oliveira R, Teixeira MM: Chemokine receptor expression on the surface of peripheral blood mononuclear cells in Chagas disease. J Infect Dis. 2004, 189 (2): 214-220. 10.1086/380803.
Araujo FF, Gomes JA, Rocha MO, Williams-Blangero S, Pinheiro VM, Morato MJ, Correa-Oliveira R: Potential role of CD4 + CD25HIGH regulatory T cells in morbidity in Chagas disease. Front Biosci. 2007, 12: 2797-2806. 10.2741/2273.
Guedes PM, Gutierrez FR, Silva GK, Dellalibera-Joviliano R, Rodrigues GJ, Bendhack LM, Rassi A, Rassi A, Schmidt A, Maciel BC, et al: Deficient regulatory T cell activity and low frequency of IL-17-producing T cells correlate with the extent of cardiomyopathy in human Chagas’ disease. PLoS Negl Trop Dis. 2012, 6 (4): e1630-10.1371/journal.pntd.0001630.
Magalhaes LM, Villani FN, Nunes Mdo C, Gollob KJ, Rocha MO, Dutra WO: High interleukin 17 expression is correlated with better cardiac function in human Chagas disease. J Infect Dis. 2013, 207 (4): 661-665. 10.1093/infdis/jis724.
Abel LC, Rizzo LV, Ianni B, Albuquerque F, Bacal F, Carrara D, Bocchi EA, Teixeira HC, Mady C, Kalil J, et al: Chronic Chagas’ disease cardiomyopathy patients display an increased IFN-gamma response to Trypanosoma cruzi infection. J Autoimmun. 2001, 17 (1): 99-107. 10.1006/jaut.2001.0523.
Reis DD, Jones EM, Tostes S, Lopes ER, Chapadeiro E, Gazzinelli G, Colley DG, McCurley TL: Expression of major histocompatibility complex antigens and adhesion molecules in hearts of patients with chronic Chagas’ disease. Am J Trop Med Hyg. 1993, 49 (2): 192-200.
Nogueira LG, Santos RH, Ianni BM, Fiorelli AI, Mairena EC, Benvenuti LA, Frade A, Donadi E, Dias F, Saba B, et al: Myocardial Chemokine expression and intensity of myocarditis in Chagas cardiomyopathy Are controlled by polymorphisms in CXCL9 and CXCL10. PLoS Negl Trop Dis. 2012, 6 (10): e1867-10.1371/journal.pntd.0001867.
Gomes JA, Bahia-Oliveira LM, Rocha MO, Busek SC, Teixeira MM, Silva JS, Correa-Oliveira R: Type 1 chemokine receptor expression in Chagas’ disease correlates with morbidity in cardiac patients. Infect Immun. 2005, 73 (12): 7960-7966. 10.1128/IAI.73.12.7960-7966.2005.
Zicker F, Smith PG, Netto JC, Oliveira RM, Zicker EM: Physical activity, opportunity for reinfection, and sibling history of heart disease as risk factors for Chagas’ cardiopathy. Am J Trop Med Hyg. 1990, 43 (5): 498-505.
Llop E, Rothhammer F, Acuna M, Apt W: HLA antigens in cardiomyopathic Chilean chagasics. Am J Hum Genet. 1988, 43 (5): 770-773.
Llop E, Rothhammer F, Acuna M, Apt W, Arribada A: HLA antigens in Chagas cardiomyopathy: new evidence based on a case–control study. Rev Med Chil. 1991, 119 (6): 633-636.
Fernandez-Mestre MT, Layrisse Z, Montagnani S, Acquatella H, Catalioti F, Matos M, Balbas O, Makhatadze N, Dominguez E, Herrera F, et al: Influence of the HLA class II polymorphism in chronic Chagas’ disease. Parasite Immunol. 1998, 20 (4): 197-203.
Layrisse Z, Fernandez MT, Montagnani S, Matos M, Balbas O, Herrera F, Colorado IA, Catalioti F, Acquatella H: HLA-C(*)03 is a risk factor for cardiomyopathy in Chagas disease. Hum Immunol. 2000, 61 (9): 925-929. 10.1016/S0198-8859(00)00161-0.
Deghaide NH, Dantas RO, Donadi EA: HLA class I and II profiles of patients presenting with Chagas’ disease. Dig Dis Sci. 1998, 43 (2): 246-252. 10.1023/A:1018829600200.
Colorado IA, Acquatella H, Catalioti F, Fernandez MT, Layrisse Z: HLA class II DRB1, DQB1, DPB1 polymorphism and cardiomyopathy due to Trypanosoma cruzi chronic infection. Hum Immunol. 2000, 61 (3): 320-325. 10.1016/S0198-8859(99)00177-9.
Rodriguez-Perez JM, Cruz-Robles D, Hernandez-Pacheco G, Perez-Hernandez N, Murguia LE, Granados J, Reyes PA, Vargas-Alarcon G: Tumor necrosis factor-alpha promoter polymorphism in Mexican patients with Chagas’ disease. Immunol Lett. 2005, 98 (1): 97-102. 10.1016/j.imlet.2004.10.017.
Campelo V, Dantas RO, Simoes RT, Mendes-Junior CT, Sousa SM, Simoes AL, Donadi EA: TNF microsatellite alleles in Brazilian Chagasic patients. Dig Dis Sci. 2007, 52 (12): 3334-3339. 10.1007/s10620-006-9699-7.
Drigo SA, Cunha-Neto E, Ianni B, Mady C, Fae KC, Buck P, Kalil J, Goldberg AC: Lack of association of tumor necrosis factor-alpha polymorphisms with Chagas disease in Brazilian patients. Immunol Lett. 2007, 108 (1): 109-111. 10.1016/j.imlet.2006.10.008.
Beraun Y, Nieto A, Collado MD, Gonzalez A, Martin J: Polymorphisms at tumor necrosis factor (TNF) loci are not associated with Chagas’ disease. Tissue Antigens. 1998, 52 (1): 81-83. 10.1111/j.1399-0039.1998.tb03028.x.
Drigo SA, Cunha-Neto E, Ianni B, Cardoso MR, Braga PE, Fae KC, Nunes VL, Buck P, Mady C, Kalil J, et al: TNF gene polymorphisms are associated with reduced survival in severe Chagas’ disease cardiomyopathy patients. Microbes Infect. 2006, 8 (3): 598-603. 10.1016/j.micinf.2005.08.009.
Florez O, Martin J, Gonzalez CI: Interleukin 4, interleukin 4 receptor-alpha and interleukin 10 gene polymorphisms in Chagas disease. Parasite Immunol. 2011, 33 (9): 506-511. 10.1111/j.1365-3024.2011.01314.x.
Cruz-Robles D, Chavez-Gonzalez JP, Cavazos-Quero MM, Perez-Mendez O, Reyes PA, Vargas-Alarcon G: Association between IL-1B and IL-1RN gene polymorphisms and Chagas’ disease development susceptibility. Immunol Invest. 2009, 38 (3–4): 231-239.
Costa GC, da Costa Rocha MO, Moreira PR, Menezes CA, Silva MR, Gollob KJ, Dutra WO: Functional IL-10 gene polymorphism is associated with Chagas disease cardiomyopathy. J Infect Dis. 2009, 199 (3): 451-454. 10.1086/596061.
Moreno M, Silva EL, Ramirez LE, Palacio LG, Rivera D, Arcos-Burgos M: Chagas’ disease susceptibility/resistance: linkage disequilibrium analysis suggests epistasis between major histocompatibility complex and interleukin-10. Tissue Antigens. 2004, 64 (1): 18-24. 10.1111/j.1399-0039.2004.00260.x.
Zafra G, Morillo C, Martin J, Gonzalez A, Gonzalez CI: Polymorphism in the 3′ UTR of the IL12B gene is associated with Chagas’ disease cardiomyopathy. Microbes Infect. 2007, 9 (9): 1049-1052. 10.1016/j.micinf.2007.04.010.
Ramasawmy R, Cunha-Neto E, Fae KC, Borba SC, Teixeira PC, Ferreira SC, Goldberg AC, Ianni B, Mady C, Kalil J: Heterozygosity for the S180L variant of MAL/TIRAP, a gene expressing an adaptor protein in the Toll-like receptor pathway, is associated with lower risk of developing chronic Chagas cardiomyopathy. J Infect Dis. 2009, 199 (12): 1838-1845. 10.1086/599212.
Ramasawmy R, Cunha-Neto E, Fae KC, Martello FG, Muller NG, Cavalcanti VL, Ianni B, Mady C, Kalil J, Goldberg AC: The monocyte chemoattractant protein-1 gene polymorphism is associated with cardiomyopathy in human chagas disease. Clin Infect Dis. 2006, 43 (3): 305-311. 10.1086/505395.
Ramasawmy R, Cunha-Neto E, Fae KC, Muller NG, Cavalcanti VL, Drigo SA, Ianni B, Mady C, Kalil J, Goldberg AC: BAT1, a putative anti-inflammatory gene, is associated with chronic Chagas cardiomyopathy. J Infect Dis. 2006, 193 (10): 1394-1399. 10.1086/503368.
Ramasawmy R, Fae KC, Cunha-Neto E, Muller NG, Cavalcanti VL, Ferreira RC, Drigo SA, Ianni B, Mady C, Goldberg AC, et al: Polymorphisms in the gene for lymphotoxin-alpha predispose to chronic Chagas cardiomyopathy. J Infect Dis. 2007, 196 (12): 1836-1843. 10.1086/523653.
Pissetti CW, de Oliveira RF, Correia D, Nascentes GA, Llaguno MM, Rodrigues V: Association between the lymphotoxin-alpha gene polymorphism and chagasic cardiopathy. J Interferon Cytokine Res. 2013, 33 (3): 130-135. 10.1089/jir.2012.0024.
Ramasawmy R, Fae KC, Cunha-Neto E, Borba SC, Ianni B, Mady C, Goldberg AC, Kalil J: Variants in the promoter region of IKBL/NFKBIL1 gene may mark susceptibility to the development of chronic Chagas’ cardiomyopathy among Trypanosoma cruzi-infected individuals. Mol Immunol. 2008, 45 (1): 283-288. 10.1016/j.molimm.2007.04.015.
Calzada JE, Nieto A, Beraun Y, Martin J: Chemokine receptor CCR5 polymorphisms and Chagas’ disease cardiomyopathy. Tissue Antigens. 2001, 58 (3): 154-158. 10.1034/j.1399-0039.2001.580302.x.
Fernandez-Mestre MT, Montagnani S, Layrisse Z: Is the CCR5-59029-G/G genotype a protective factor for cardiomyopathy in Chagas disease?. Hum Immunol. 2004, 65 (7): 725-728. 10.1016/j.humimm.2004.05.002.
Torres OA, Calzada JE, Beraun Y, Morillo CA, Gonzalez CI, Gonzalez A, Martin J: Association of the macrophage migration inhibitory factor -173G/C polymorphism with Chagas disease. Hum Immunol. 2009, 70 (7): 543-546. 10.1016/j.humimm.2009.04.022.
Torres OA, Calzada JE, Beraun Y, Morillo CA, Gonzalez A, Gonzalez CI, Martin J: Role of the IFNG +874 T/A polymorphism in Chagas disease in a Colombian population. Infect Genet Evol. 2010, 10 (5): 682-685. 10.1016/j.meegid.2010.03.009.
Cunha-Neto E, Kalil J: Autoimmunity in Chagas’ heart disease. Sao Paulo Med J. 1995, 113 (2): 757-766.
Teixeira PC, Frade AF, Nogueira LG, Kalil J, Chevillard C, Cunha-Neto E: Pathogenesis of Chagas disease cardiomyopathy. World J Clin Infect Dis. 2012, 2 (3): 39-53. 10.5495/wjcid.v2.i3.39.
Vasconcelos RH, Montenegro SM, Azevedo EA, Gomes YM, Morais CN: Genetic susceptibility to chronic Chagas disease: an overview of single nucleotide polymorphisms of cytokine genes. Cytokine. 2012, 59 (2): 203-208. 10.1016/j.cyto.2012.04.035.
Fae KC, Drigo SA, Cunha-Neto E, Ianni B, Mady C, Kalil J, Goldberg AC: HLA and beta-myosin heavy chain do not influence susceptibility to Chagas disease cardiomyopathy. Microbes Infect. 2000, 2 (7): 745-751. 10.1016/S1286-4579(00)00501-3.
Weitzel T, Zulantay I, Danquah I, Hamann L, Schumann RR, Apt W, Mockenhaupt FP: Mannose-binding lectin and Toll-like receptor polymorphisms and Chagas disease in Chile. Am J Trop Med Hyg. 2012, 86 (2): 229-232. 10.4269/ajtmh.2012.11-0539.
Jorge MT, Macedo TA, Janones RS, Carizzi DP, Heredia RA, Acha RE: Types of arrhythmia among cases of American trypanosomiasis, compared with those in other cardiology patients. Ann Trop Med Parasitol. 2003, 97 (2): 139-148. 10.1179/000349803235001561.
van Veldhuisen DJ, Linssen GC, Jaarsma T, van Gilst WH, Hoes AW, Tijssen JG, Paulus WJ, Voors AA, Hillege HL: B-type natriuretic peptide and prognosis in heart failure patients with preserved and reduced ejection fraction. J Am Coll Cardiol. 2013, 61 (14): 1498-1506. 10.1016/j.jacc.2012.12.044.
Januzzi JL: Natriuretic peptides, ejection fraction, and prognosis: parsing the phenotypes of heart failure. J Am Coll Cardiol. 2013, 61 (14): 1507-1509. 10.1016/j.jacc.2013.01.039.
Barretto AC, Arteaga E, Mady C, Ianni BM, Bellotti G, Pileggi F: Male sex. Prognostic factor in Chagas’ disease. Arq Bras Cardiol. 1993, 60 (4): 225-227.
Basquiera AL, Sembaj A, Aguerri AM, Omelianiuk M, Guzman S, Moreno Barral J, Caeiro TF, Madoery RJ, Salomone OA: Risk progression to chronic Chagas cardiomyopathy: influence of male sex and of parasitaemia detected by polymerase chain reaction. Heart. 2003, 89 (10): 1186-1190. 10.1136/heart.89.10.1186.
Florez O, Martin J, Gonzalez CI: Genetic variants in the chemokines and chemokine receptors in Chagas disease. Hum Immunol. 2012, 73 (8): 852-858. 10.1016/j.humimm.2012.04.005.
Marino AP, da Silva A, dos Santos P, Pinto LM, Gazzinelli RT, Teixeira MM, Lannes-Vieira J: Regulated on activation, normal T cell expressed and secreted (RANTES) antagonist (Met-RANTES) controls the early phase of Trypanosoma cruzi-elicited myocarditis. Circulation. 2004, 110 (11): 1443-1449. 10.1161/01.CIR.0000141561.15939.EC.
Medeiros GA, Silverio JC, Marino AP, Roffe E, Vieira V, Kroll-Palhares K, Carvalho CE, Silva AA, Teixeira MM, Lannes-Vieira J: Treatment of chronically Trypanosoma cruzi-infected mice with a CCR1/CCR5 antagonist (Met-RANTES) results in amelioration of cardiac tissue damage. Microbes Infect. 2009, 11 (2): 264-273. 10.1016/j.micinf.2008.11.012.
Marino AP, Silva AA, Santos PV, Pinto LM, Gazinelli RT, Teixeira MM, Lannes-Vieira J: CC-chemokine receptors: a potential therapeutic target for Trypanosoma cruzi-elicited myocarditis. Mem Inst Oswaldo Cruz. 2005, 100 (Suppl 1): 93-96.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/13/587/prepub
This work was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM), Aix-Marseille University (Direction des Relations Internationales), the USP-COFECUB program, the ARCUS II PACA Brésil program, CNPq (the Brazilian National Research Council), and FAPESP (São Paulo State Research Funding Agency-Brazil). ECN and CC were recipient for an international program funded either by the French ANR and the Brazilian FAPESP agencies. AFF MB and LGN hold fellowships from the São Paulo State Research Funding Agency, FAPESP. ECN and JK have received a Council for Scientific and Technological Development - CNPq productivity award. CC is a recipient of a temporary professor position supported by the French consulate in Brazil and the University of São Paulo (USP). We thank for the access to the genotyping platform (Denis Milan, Cecile Donnadieu, and Frederic Martins).
The authors declare that they have no competing interests.
Contribution to conception and design: JK, ACP, ECN, CC. Performed the experiments: AFF, MB. Analysis of the data: AFF PCT ECN CC. Contributed reagents materials analysis tools: CWP, BMI, BS, HTLW, LGN, ADMB, PB, FD, AS, ED, JAMN, MH, MS, AF, VR, ACP. Wrote the paper: ECN, CC. Review the drafts: ECN, CC, AFF, LRPF. All authors read and approved the final manuscript.
Edecio Cunha-Neto and Christophe Chevillard contributed equally to this work.