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  • Open Access

Cross-clade neutralization potential of the plasma of antiretroviral naïve HIV-1 infected children from north India

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  • 1Email author
BMC Infectious Diseases201212 (Suppl 1) :P87

https://doi.org/10.1186/1471-2334-12-S1-P87

  • Published:

Keywords

  • Viral Load
  • Spearman Rank Correlation
  • Neutralize Antibody
  • Indian Child
  • Spearman Rank Correlation Test

Background

In this cross-sectional study, we evaluated the efficiency of the plasma of HIV-1 infected children from north India against a standard panel of pseudoviruses.

Methods

We recruited 38 antiretroviral naïve HIV-1 infected children after getting written informed consent from their parents/guardians. The study was approved by the institute ethics committee. Neutralization efficiency of the patients’ plasma was tested against tier 2 pseudoviruses (3 clade C - ZM53, Du172.17 and Du156.12 and 3 clade B - RHPA4259.7, TRO.11 and SC422661.8) obtained from NIH AIDS Research and Reference Reagent Program by TZM-bl assay. The inhibitory dilution at 50% neutralization (ID50 titers) was determined by non-linear regression by the method of least squares. Correlation tests were carried out using Spearman rank correlation test.

Results

The median age of the children was six years (range 1.5-14). The median viral load was 24000 RNA copies/ml (range <47-585000) and CD4 count was 655 cells/µl (range 131-2458). Cross neutralization was observed in 28.9% (11/38) of the children. Clade specific neutralization was observed in 47.4% (18/38) against clade C and 7.9% (3/38) against clade B while 15.8% (6/38) of the children did not show neutralization against any of the viruses. There was a significant positive correlation between viremia and neutralization efficiency against two of the viruses studied (Du172 r=0.49; p=0.007 and RHPA r=0.47; p=0.01).

Conclusion

This is the first report on the neutralization efficiency of the plasma of HIV-1 infected Indian children against tier 2 pseudoviruses. Cross-clade neutralizing antibodies were observed in 29% of them.

Authors’ Affiliations

(1)
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, Delhi, 110029, India

Copyright

© Sankaran et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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