Nitric Oxide (NO) and superoxide production by macrophages after infection with
L. braziliensis. Six-day monocyte derived-macrophages from HS (n = 6), SC (n = 6), CL (n = 6) and ML (n = 05) groups were infected or left uninfected with L. braziliensis at a 2:1 ratio. After 48 and 96 h, the supernatants were collected for detection of NO (A) and superoxide (B) using the Griess reaction. The data are expressed as the mean ± SD. Statistical analysis was performed using the Kruskal-Wallis test. (p > 0.05).