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Table 2 Laboratory methods for identification and susceptibility testing

From: Bacterial infections in Lilongwe, Malawi: aetiology and antibiotic resistance

Organism Media Tests for presumptive identification Susceptibility panel Method
Salmonellae BAP Gram stain; GNP Disc diffusion
  MAC TSI;   
   Indole;   
   Colony morphology;   
   Urease   
Enteric gram negative bacilli BAP Gram stain; GNP Disc diffusion
  MAC Colony morphology   
   Indole;   
   Oxidase;   
   TSI;   
   Citrate;   
   Urease   
N. meningitidis BAP Gram stain None None
  CHOC Oxidase   
  TM Thayer-Martin   
S. pneumoniae BAP Gram stain GPP Disc diffusion
  CHOC Hemolysis Catalase Oxacillin  
   Bile solubility screening test  
S. aureus BAP Gram stain GPP Disc diffusion
  CHOC Catalase Oxacillin  
  MSA Coagulase   
   Staphlex   
C. neoformans Sabouraud's Gram stain None None
  BAP India Ink   
   Urease   
Haemophilus BAP Gram stain None None
  CHOC Oxidase   
   API-NH   
S. pyogenes BAP Haemolysis GPP Disc diffusion
   Gram stain   
  1. BAP = blood agar plate, MAC = MacConkey agar, CHOC = chocolate agar, TSI = triple-sugar with iron media, TM = Thayer-Martin agar, MSA = mannitol salt agar. Coagulase = tube coagulate test using human plasma; GPP = gram positive antibiotic panel, GNP = gram negative antibiotic panel, see Table 3.