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Table 4 Evaluation of different TRF assays using anti-HIV-1 low titer performance panel (PRB 108)

From: Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Member ID # Abbott HIV 1/2a Gen. Sys. HIV 1/2a Org. Tek. HIV 1a Bio-Rad HIV-1 Western Blot Resultb Roche PCR HIV-1 RNA test copies/mlc TRF assaysd
r-Δgp160 two-step r-Δgp160 one-step r-HIV-1env two-step
1 5.5 >8.9 1.4 + 3x104 1.9 (+) 1.8 (+) 2.0 (+)
2 0.2 0.3 0.3 - BLD 0.8 (−) 0.6 (−) 0.7 (−)
3e NA NA 1.8 IND 5x101* 3.4 (+) 3.0 (+) 0.8 (−)
4e 11.9 >8.9 6.3 + 9x104 7.0 (+) 5.2 (+) 3.4 (+)
5 3.4 >8.9 3.2 + 5x101* 2.2 (+) 1.2 (+) 3.6 (+)
6 NA NA 1.2 IND 6x103 1.8 (+) 1.1 (+) 5.9 (+)
7f 5.3 >8.9 1.3 + 3x103 2.7 (+) 1.6 (+) 3.9 (+)
8f 8.0 >8.9 2.8 + 7x103 3.8 (+) 2.8 (+) 4.8 (+)
9 8.5 >8.9 0.7 + 2x103 1.9 (+) 1.1 (+) 7.2 (+)
10 8.6 >8.9 0.6 IND 3x105 1.7 (+) 1.2 (+) 13.2 (+)
11 NA NA 3.8 + 8x103 3.2 (+) 2.0 (+) 5.4 (+)
12 1.7 2.0 0.3 - 5x105 1.1 (+) 0.6 (−) 0.9 (−)
13 NA NA 0.9 IND 2x105 4.9 (+) 2.7 (+) 18.2 (+)
14g NA NA 0.4 - 3x105 1.4 (+) 1.0 (+) 1.1 (+)
15g 14.2 >8.9 1.8 IND >7x105 5.9 (+) 3.0 (+) 8.0 (+)
  1. aValues indicate S/Co ratios, provided by the panel supplier using the indicated commercial EIA kits. S/Co values ≥ 1.0 are considered as positive. (Gen. Sys., Genetic Systems; Org.Tek., Organon Teknika). ‘NA’ indicates data ‘not available’ from the panel supplier.
  2. bResult provided by the panel supplier using the indicated commercial test. ‘+’ and ‘–’ indicate the presence and absence, respectively, of antigen bands in the Western blot assay. ‘IND’ indicates indeterminate result.
  3. CResult provided by the panel supplier using the indicated commercial test. ‘BLD’ indicates that the RNA level is below the limit of detection. *Ultrasensitive method was used (50–75,000 copies/ml).
  4. dValues indicate S/Co ratios obtained in this study, using the in-house TRF assays. The results are indicated in parentheses. Samples with S/Co values < 1.0 are designated as negative (−) and those with values ≥ 1.0 are designated as positive (+).
  5. ePRB 108–4 is a bleed from the same donor as PRB 108–3 collected 17 months later.
  6. fPRB 108–8 is a bleed from the same donor as PRB 108–7 collected 6 days later.
  7. gPRB 108–15 is a bleed from the same donor as PRB 108–14 collected 7 days later.