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Table 3 Evaluation of r-Δgp160-based TRF assays using viral co-infection performance panel (PCA 201)

From: Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Member ID# Ortho EIA HCVa Abbott EIA HBsAga OT Anti-HBca HTLV HIV-1 TRF assaysb
Abbott EIAa GS Blotsa Abbott EIAa Dupont blotsa r-Δgp160 two-step r-Δgp160 one-step
1 + + + + P 0.2 N/A 0.9 (−) 0.6 (−)
2 - + + - N/A 13.5 P 96.5 (+) 6.5 (+)
3 + + + + P 0.1 N/A 0.5 (−) 0.7 (−)
4 - + + + P 0.1 N/A ND ND
5 + - + + P 13.5 P 122.6 (+) 8.5 (+)
6 - - - - N/A 0.1 N/A ND ND
7 + - - + P 13.5 P 146.1 (+) 9.0 (+)
8 + + + - N/A 13.5 P 208.4 (+) 5.2 (+)
9 + + - + P 13.5 P 107.7 (+) 8.5 (+)
10 - + + - N/A 13.5 P 84.9 (+) 13.3 (+)
11 + + + + P 0.1 N/A 0.7 (−) 0.6 (−)
12 + + + - N/A 13.5 P 142.2 (+) 1.1 (+)
13 - + + - N/A 13.5 P 47.8 (+) 11.2 (+)
14 - + + + P 0.32 N/A 0.8 (−) 0.7 (−)
15 + + + + IND 0.2 N/A 0.8 (−) 0.7 (−)
16 + - + + P 13.5 P 182.5 (+) 4.5 (+)
17 + + + - N/A 13.5 P 215.4 (+) 9.1 (+)
18 + + - + P 13.5 P 48.3 (+) 3.6 (+)
19 - + + - N/A 13.5 P 88.2 (+) 9.7 (+)
20 - + + - N/A 1.1 P 3.3 (+) 2.5 (+)
21 - + + - N/A 13.5 P 128.6 (+) 3.0 (+)
22 + + + - N/A 13.5 P 140.6 (+) 16.9 (+)
23 + + + + P 0.2 N/A 0.8 (−) 0.8 (−)
24 - - - - N/A 0.1 N/A 0.8 (−) 0.8 (−)
25 - + + - N/A 13.5 P 127.1 (+) 6.0 (+)
  1. aAssays performed by Boston Biomedica Inc (now SeraCare Life Sciences) using commercial kits. Values for Abbott anti-HIV EIA represent S/Co ratios, provided by the panel supplier. Results of other EIA tests, provided by panel supplier are indicated as either positive (‘+’, S/Co ≥ 1.0) or negative (‘-’, S/Co < 1.0) for simplicity. HTLV and HIV-1 EIA positive samples were also tested in blot formats using kits from GS (Genetic Systems) and Dupont, respectively. ‘P’and ‘IND’ indicate the positive and indeterminate results, respectively, depending upon the presence or absence of antigen bands in the blot assays. ‘N/A’ indicates not applicable. (‘OT’, Organon Teknika; ‘GS’, Genetic Systems).
  2. bValues indicate S/Co ratios obtained using r-Δgp160-based TRF assays. The results are indicated in parentheses. Samples with S/Co values < 1.0 are designated as negative (−) and those with values ≥ 1.0 are designated as positive (+). ‘ND’ indicates ‘not determined’ due to lack of sample.