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Table 2 Evaluation of r-Δgp160-based TRF assays using worldwide HIV performance panel (WWRB 302)

From: Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Member ID# Origin Gtpa Abbott Gen. Sys. OT HIV1b TRF assaysc
HIV1b HIV1/2b HIV1b HIV1/2b r-Δgp160 two-step r-Δgp160 one-step
01 Spain O 1.1 1.8 0.8 5.6 1.3 1.3 (+) 0.6 (−)
02 Ghana A >11.5 >16.1 6.9 8.7 7.0 94.0 (+) 9.2 (+)
03 Ghana G >11.5 >16.1 7.1 8.8 7.2 164.8 (+) 41.6 (+)
04 Ghana G >11.5 >16.1 7.1 8.8 6.5 138.0 (+) 4.1 (+)
05 Ghana A >11.5 >16.1 7.1 8.7 7.0 253.6 (+) 82.2 (+)
06 Ghana G >11.5 >16.1 6.9 8.8 7.1 91.4 (+) 15.1 (+)
08 Ivory Coast G >11.5 >16.1 6.9 8.7 6.7 73.0 (+) 4.4 (+)
09 Ivory Coast A >11.5 >16.1 6.9 8.6 6.5 107.8 (+) 3.9 (+)
10 Ivory Coast Neg 0.4 0.2 0.1 0.4 0.4 0.7 (−) 0.4 (−)
11 Mozambique HIV-2 1.2 14.6 0.6 9.7 3.0 1.0 (+) 0.7 (−)
12 Mozambique C >11.5 >16.1 7.1 8.9 6.9 187.1 (+) 4.1 (+)
14 Uganda D >11.5 >16.1 4.5 8.5 6.2 53.3 (+) 13.8 (+)
15 Uganda D >11.5 >16.1 6.3 8.1 7.2 132.9 (+) 16.8 (+)
16 Uganda D >11.5 >16.1 7.0 8.8 6.9 154.5 (+) 7.3 (+)
17 Uganda D >11.5 >16.1 6.8 9.8 7.0 131.2 (+) 5.1 (+)
19 Zimbabwe C >11.5 >16.1 6.0 9.9 7.0 41.4 (+) 10.3 (+)
21 China B >11.5 >16.1 6.7 8.8 7.0 136.7 (+) 26.8 (+)
22 Thailand E >11.5 >16.1 7.3 9.8 7.0 100.8 (+) 7.0 (+)
24 Thailand E >11.5 >16.1 7.4 9.8 6.9 88.4 (+) 23.2 (+)
25 India HIV-2 0.4 15.4 3.8 10 2.1 4.9 (+) 1.5 (+)
26 USA D >11.5 >16.1 7.4 9.8 7.1 85.8 (+) 48.5 (+)
27 USA B/D >11.5 >16.1 7.0 9.8 7.2 135.7 (+) 12.7 (+)
28 Argentina F >11.5 >16.1 7.0 8.9 6.8 72.5 (+) 3.0 (+)
29 Argentina B >11.5 >16.1 6.9 8.5 6.6 92.9 (+) 2.2 (+)
30 Argentina Neg 0.3 0.2 0.2 0.2 0.4 0.9 (−) 0.4 (−)
  1. aGenotype.
  2. bValues indicate S/Co ratios, provided by the panel supplier using the indicated commercial EIA kits. S/Co values ≥ 1.0 are considered as positive. (Gen. Sys., Genetic Systems; OT, Organon Teknika).
  3. cValues indicate S/Co ratios obtained using r-Δgp160-based TRF assays. The results are indicated in parentheses. Samples with S/Co values < 1.0 are designated as negative (−) and those with values ≥ 1.0 are designated as positive (+).