You are viewing the site in preview mode

Skip to main content

Advertisement

Table 1 Evaluation of r-Δgp160-based TRF assays using HIV-1 seroconversion panel (PRB 931)

From: Escherichia coli–expressed near full length HIV-1 envelope glycoprotein is a highly sensitive and specific diagnostic antigen

Member ID# Days since 1stbleed Abbott Gen. Sys. Organon Teknika HIVa TRF assaysb
HIV1a HIV1/2a HIV1a HIV1/2a r-Δgp160 two-step r-Δgp160 one-step
01 0 0.2 0.1 0.2 0.1 0.3 0.9 (−) 0.5 (−)
02 2 0.2 0.1 0.1 0.1 0.3 0.8 (−) 0.5 (−)
03 7 0.2 0.1 0.2 0.1 0.4 0.8 (−) 0.4 (−)
04 9 0.2 0.1 0.2 0.1 0.3 0.8 (−) 0.5 (−)
05 15 0.2 0.1 0.2 0.1 0.3 0.7 (−) 0.5 (−)
06 28 0.9 6 0.3 0.4 0.6 17.2 (+) 5.6 (+)
07 33 3.9 >18.7 0.8 1.1 2.3 15.9 (+) 4.3 (+)
08 35 5.7 >18.7 1.3 1.9 3.1 11.9 (+) 3.3 (+)
09 42 10.5 >18.7 2.9 4 4.6 13.8 (+) 2.9 (+)
  1. aValues indicate S/Co ratios, provided by the panel supplier using the indicated commercial EIA kits. S/Co values ≥ 1.0 are considered as positive.
  2. bValues indicate S/Co ratios obtained using r-Δgp160-based TRF assays. The results are indicated in parentheses. Samples with S/Co values < 1.0 are designated as negative (−) and those with values ≥ 1.0 are designated as positive (+).