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Emergence of serogroup C meningococcal disease associated with a high mortality rate in Hefei, China
© Xu et al.; licensee BioMed Central Ltd. 2012
- Received: 15 October 2011
- Accepted: 23 August 2012
- Published: 4 September 2012
Neisseria meningitidis serogroup C has emerged as a cause of epidemic disease in Hefei. The establishment of serogroup C as the predominant cause of endemic disease has not been described.
We conducted national laboratory-based surveillance for invasive meningococcal disease during 2000–2010. Isolates were characterized by pulsed-field gel electrophoresis and multilocus sequence typing.
A total of 845 cases of invasive meningococcal disease were reported. The incidence increased from 1.25 cases per 100,000 population in 2000 to 3.14 cases per 100,000 in 2003 (p < 0.001), and peaked at 8.43 cases per 100,000 in 2005. The increase was mainly the result of an increase in the incidence of serogroup C disease. Serogroup C disease increased from 2/23 (9%) meningococcal cases and 0.11 cases per 100,000 in 2000 to 33/58 (57%) cases and 1.76 cases per 100,000 in 2003 (p < 0.01). Patients infected with serogroup C had serious complications more frequently than those infected with other serogroups. Specifically, 161/493 (32.7%) cases infected with serogroup C had at least one complication. The case-fatality rate of serogroup C meningitis was 11.4%, significantly higher than for serogroup A meningitis (5.3%, p = 0.021). Among patients with meningococcal disease, factors associated with death in univariate analysis were age of 15–24 years, infection with serogroup C, and meningococcemia.
The incidence of meningococcal disease has substantially increased and serogroup C has become endemic in Hefei. The serogroup C strain has caused more severe disease than the previously predominant serogroup A strain.
- Neisseria meningitidis
- Serogroup C strain
Meningococcal disease is an important cause of meningitis and sepsis in children and young adults in China and worldwide. In China, this disease previously occurred in a cyclical pattern at intervals of 8–10 years . Nationwide epidemics occurred in 1959, 1967, 1977, and 1984 [2, 3]. Since a national immunisation campaign with meningococcal A polysaccharide vaccine was initiated in 1982, no countrywide epidemic has occurred, and morbidity rates of meningococcal disease in China have remained relatively stable at 0.2–1 cases per 100,000 during the past two decades [3–5]. During this time, serogroup A meningococci was most common serogroup to cause disease and was responsible for more than 95% of cases, whereas serogroups B cause only sporadic cases and serogroup C was even rarer [4, 5]. However, since 2003 the number of meningococcal meningitis cases attributable to serogroup C has increased substantially in China . The sudden increase in the number of cases due to serogroup C meningococci first occurred in China’s Anhui Province during 2003–2004. Hefei, the capital of Anhui Province—a region situated in the middle China with a population of 1.85 million in 2006—had one of the first Chinese outbreaks of meningococcal disease caused by serogroup C . The incidence of meningococcal disease due to serogroup C increased rapidly, and Neisseria meningitidis serogroup C emerged as a new epidemic serogroup in Hefei and other areas of Anhui Province . To control this disease, mass vaccination using polysaccharide vaccine for serogroup A plus C in students and children younger than 6 years was undertaken starting in 2004 in Anhui Province  Chemoprophylaxis was given to close contacts of patients, and a nationwide surveillance programme was implemented . No epidemics of meningococcal disease caused by serogroup C occurred in China before 2002, and few reports on its epidemiological characteristics in China have been published . In this article, we report the replacement of serogroup A disease by serogroup C in Hefei, China. This led to an increased incidence of disease among young infants and higher case fatality rates in the general population.
Case reporting and laboratory procedures
The protocol of the study adhered to the tenets of the Declaration of Helsinki and was approved by the ethics committee of Anhui Medical University. In the National Notifiable Disease Surveillance System in Hefei, patients with sudden onset of fever, headache, nausea, vomiting, stiff neck, petechial rash, accompanied by delirium, coma or shock, or Gram negative diplococci found in a smear of cerebrospinal fluid (CSF) by Gram stain should be reported within six hours to the Center for Disease Control (CDC), Hefei as suspected cases of invasive meningococcal disease. In addition to routine examination and culture in each hospital, blood and/or CSF from the suspected N. meningitidis patient was sent at room temperature to the bacteriology laboratory of Hefei CDC as soon as possible for bacterial culture and serogroup classification. Within 12 hours of the suspected case being reported, staff at the local Health Bureau conducted a case investigation to understand the detailed travel history and identify every close contact during the two weeks before disease onset. If N. meningitidis was isolated and the patient had compatible clinical symptoms and signs, the patient was a confirmed case of invasive meningococcal disease. The identification of all isolates were confirmed at Hefei CDC using conventional biochemical methods . An agglutination test (Murex Biotech Ltd, Dartford, UK) for standard serogroup testing was also performed at Hefei CDC. Serogroups were determined by slide agglutination with polyclonal antibodies to capsular polysaccharides A, C, X, Y, Z, and W135 and monoclonal antibodies to polysaccharide B.
The minimal inhibitory concentrations (MICs) of the antimicrobial agents were determined for each isolate by Mueller-Hinton agar (OXOID Company, Cambridge, UK) macrodilution method described by the CLSI (formerly the National Committee for Clinical Laboratory Standards) . The MIC was defined as the lowest concentration of drug that resulted in no visible growth after 18 h of incubation at 35°C in ambient air. Quality control strains were included with every batch of clinical isolates to ensure accuracy and comparable performance of the assays.
Cases were defined as those from which N. meningitidis was isolated from normally sterile specimens (e.g., CSF, blood, or joint fluid specimens). In addition, in 2004 the definition for case patients was broadened to include patients with culture-negative specimens that yielded positive results by latex agglutination and Gram stain microscopy or by latex agglutination and polymerase chain reaction. Bacteria were identified according to standardized procedures . Laboratory-confirmed meningococcal meningitis was defined as growth of N. meningitidis on CSF cultures (with or without growth on blood cultures). Laboratory-confirmed meningococcemia was defined as growth of N. meningitidis on blood cultures (without growth on CSF cultures).
Clinical information collection
We reviewed the available medical information of all cases reported to Hefei CDC from 1 January, 2000 to 30 December, 2010. The captured data included patients' history, clinical manifestations and complications, laboratory results, therapy, and outcome. Patients were categorized as either meningitis or meningococcemia.
We calculated the incidence based on the number of laboratory-confirmed cases reported each year from 1 January through 31 December divided by midyear population estimates for each year, as supplied by Statistics Hefei. In 2006, the estimated population of Hefei was 1.85 million.
Serogroup C disease was compared with serogroup A disease alone, because serogroup A has specific epidemiological features and had previously been predominant in Hefei. The prevalence of other serogroups in the area did not vary over time and caused minimal disease. Univariate assessments of characteristics associated with disease due to serogroup C infection and disease resulting in death were performed using Fisher’s exact test or the Mantel-Haenszel test for categorical variables. Variables available for evaluation as potential risk factors included age group, sex, and syndrome (laboratory confirmed meningitis versus meningococcemia). Multivariable logistic regression models were fitted to the data, starting with all variables that were significant at p < 0.05 on univariate analysis and dropping non-significant factors with stepwise backward selection. Independence of data was assumed, because the vast majority of cases were considered to be unrelated. The criterion of significance was p < 0.05 in a two-tailed test. Statistical analyses were performed using SPSS V11.0 software (SPSS Inc, 2000).
Number and incidence of laboratory-confirmed invasive meningococcal cases in Hefei reported to The Hefei CDC, by serogroup, 2000–2010
Year of surverillance
Of the meningococcal strains that were isolated during this study we analyzed 374 for drug resistance. Sixty-four (17%) meningococcal isolates were not susceptible to penicillin. The proportion of resistant isolates fluctuated by year, from 2/17 (11.8%) isolates in 2000 to a peak of 16/78 (20.5%) isolates in 2005. Penicillin-resistant isolates of all serogroups were found. Nine of 97 (9.3%) serogroup A isolates and 51/264 (19.3%) serogroup C strains (p = 0.023) were not susceptible to penicillin. The rate of trimeth-sulfa resistant strains was below 25% in serogroup A cases and above 50% in serogroup C cases. All 374 isolates were susceptible to ceftriaxone, cefotaxime and cefoxitin, and only 10 isolates were resistant to rifampin.
Age, sex, and seasonal distribution
Of the 792 serogroup C and serogroup A cases, 444 (56.1%) were male and 348 (43.9%) were female. The distribution showed no significant difference between the two groups (Mann–Whitney U test: p = 0.33).
The monthly distribution of patients shows that the lowest incidence is in the summer months and the highest incidence is in the late winter months (data not shown). There were no statistically significant differences in seasonal characteristics between cases infected with serogroup C and serogroup A (data not shown).
Comparison of clinical features between serogroup C and serogroup A cases
Clinical manifestations, laboratory data, and outcome of 670 meningococcal disease patients with completed case records: serogroup C versus A
Serogroup C (n = 493)
Serogroup A (n = 177)
Presentation days (mean ± SD)
1.8 ± 2.5
1.9 ± 2.4
Nausea and/or vomiting
Petechia and/or purpura
Altered mental status
>10 × 109/L
10~4 × 109/L
<4 × 109/L
>100 × 109/L
100~50 × 109/L
100~50 × 109/L
CSF data (mean ± SD)
2.07 ± 1.7
1.91 ± 1.8
CSF glucose / blood glucose ratio
0.24 ± 0.23
0.21 ± 0.29
Total protein level(g/L)
3.41 ± 2.9
3.17 ± 2.18
10.4 ± 9.2
11.0 ± 8.9
5.3 ± 13.8
8.1 ± 13.6
sensorineural hearing loss
There was adequate information to objectively assess sequelae in 655 survivors. About nine percent of serogroup C cases (46 of 484 cases) and 4% of serogroup A cases (6 of 171) were affected (p = 0.013). For survivors of serogroup C disease, skin scars (3.9%) and amputations (2.9%) were the most frequent problems observed. Other problems of serogroup C survivors were permanent minor reduction in kidney function (n = 3) permanent knee damage following septic arthritis causing mild reduction in mobility (n = 2), partial paralysis (n = 1), and speech problem (n = 1). One survivor of serogroup C disease developed multiple neural deficits following an intracerebral hemorrhage.
Univariate and multivariable analyses of risk factors for death in serogroup C and A meningococcal infection in Hefei, 2000–2010
Mortality rate, no. of deaths/no. of patients (%)
OR (95% CI)
OR (95% CI)
Strain’s susceptibility to penicillin
Although N. meningitidis serogroup C is a prominent serogroup in many regions of the world and has occasionally caused epidemics and frequently causes outbreaks , only sporadic cases and no epidemics of meningococcal disease due to serogroup C occurred in China before 2002. In this study, we report the emergence of endemic serogroup C meningococcal disease and epidemiological characteristics in China. The rate of invasive meningococcal disease in Hefei increased 2.5 times from 2000 to 2003, and the incidence peaked at 8.43 cases per 100,000 in 2005. This increase was mostly because of the emergence and increase in the incidence of serogroup C disease.
The reasons for the emergence and increase in serogroup C disease in Anhui Province and parts of China (while other potentially epidemic strains have remained relatively quiescent) are not fully understood. It is well-known that molecular mechanisms likely play an important role in the epidemiology of meningococcal disease. N. meningitidis uses several mechanisms to change its characteristics, such as antigenic structure and resistance to antibiotics. Many of these changes occur through horizontal gene transfer, by which the organism obtains large DNA sequences from other meningococcal strains or other species . Capsular switching is a genetic mechanism that allows N. meningitidis to change its capsular phenotype, permiting immunologic escape [12, 13]. N. meningitidis outbreaks can be started or sustained when this occurs. Capsular switching appears to have been responsible for an outbreak of serogroup W-135 disease during the 2000 Hajj in Mecca, Saudi Arabia. Subsequent to this outbreak, the epidemic strain spread globally and, in one example, caused an epidemic in Burkina Faso [14, 15]. Selected isolates of the serogroup C clone from Hefei and the rest of Anhui Province were found to belong to ST-4821, a unique clone of serogroup C that does not belong to any of the previously reported sequence types . For this reason, capsular switching may have been a cause for the emergence of serogroup C in Anhui Province. Published data showed that this unique ST-4821 clone was first identified in Anhui Province during 2003–04, and the same ST clones were also identified in 11 other provinces in China during 2004–2005 [7, 16, 17].
The overall case-fatality rate in Hefei tripled from 2000 to 2003. We report that the rate of case-fatality with serogroup C was 2.2 times that of serogroup A during the period covered. This was of borderline statistical significance and factors associated with death in univariate analysis were age of 15–24 years, infection with serogroup C, and meningococcemia. The results of this study confirm the severity of serogroup C disease [18, 19]. The reason for the high virulence of this particular strain of N. meningitidis is unknown, but the strain is associated with a high frequency of meningococcemia with severe complications, which was of statistical significance in multivariable analysis. Meningococcemia was also independently associated with serogroup C disease and with increased risk of death. Meningococcemia has a higher case-fatality rate than meningitis .
Few published papers discuss the clinical characteristics of serogroup C meningococcal disease [18–20]. We reviewed the detailed clinical information and found serogroup C disease more likely to cause meningococcemia, decreased platelet counts, and serious complications. Our findings provide valuable information to understand the severity of serogroup C disease. Several scoring systems have been developed for assessing the prognosis and mortality of meningococcal disease [21–24]. The Glasgow Meningococcal Septicemia Prognostic Score is a good example of a clinical prediction tool for assessing the mortality of meningococcal disease [21, 22]. Most of those scoring systems are based on easily available clinical and laboratory parameters such as age distribution, period between onset of disease and admission, absence of meningitis, presence of widespread skin lesions, hypotension, metabolic acidosis, normal C-reactive protein level, absence of leukocytosis, presence of thrombocytopenia, and hypofibrinogenemia [21–24]. However, some of the above factors (e.g., C-reactive protein and fibrinogen levels) were not routinely checked in our cases.
Of note, the age distribution of patients shifted from younger to older age categories in the Hefei area during the study period. From 1989 to 1999, 78% of cases of meningococcal disease occurred in children younger than 14 years . However, the proportion of cases in this age group decreased to 31% from 2000 to 2010, while the proportion in 15–24 year olds increased from 22% (1989–1999) to 52.6% (2000–2010). The elevated incidence of meningococcal disease among adolescents and young adults has also been noted in previous studies [15, 25]. Some reports indicated that the age distribution was not uniform for all serogroups, and disease caused by serogroup C was more likely to affect older children than disease caused by serogroup B . However, another study regarded the shift toward older age as a characteristic of a meningococcal disease epidemic, and suggested that the age distribution of the disease would return to “normal” after the epidemic . Possible explanations for the change in age group affected include: N meningitidis of serogroup C was antigenically new to the entire population; adolescents and young adults have enhanced risk factors for meningococcal transmission and invasion, such as crowding, active or passive smoking, and exposure to oral secretions. Understanding the age distribution of meningococcal disease is necessary to devise control and preventive measures such as vaccinations for high-risk populations. Because serogroup C is a vaccine-preventable strain, our findings stress the importance of further study of the age distribution of this disease. In the early days of an outbreak of serogroup C meningococcal disease (2003–2004) in Anhui Province, serogroup C vaccine was only recommended for children younger than two years. In 2005 serogroup C meningococcal conjugate vaccine was introduced to all those who were younger than 25 years, especially for those populations such as middle school students, college freshmen and service personnel. From September 2003 to July 2007, 627,000 doses of serogroup A and C vaccine were used in Hefei, providing an estimated 37–51% coverage of the population aged 2–24 years. After the immunization campaign in Hefei, the incidence of serogroup C meningococcal disease decreased. In addition, serogroup C meningococcal pharyngeal carriage has likely been reduced, which has likely led to a reduction in serogroup C meningococcal disease in the unimmunized population.
The incidence of meningococcal disease substantially increased in Hefei from 2000 through 2010. This was mainly the result of an increase in the incidence of serogroup C disease, and serogroup C has become endemic in Hefei. Patients infected with serogroup C had greater disease severity more frequently than those infected with serogroup A, the previous predominant serogroup.
The authors appreciate all those physicians providing the clinical data of the cases for analysis in this study. This study was supported by the Natural Science Foundation of China (No. 30972631).
- Su L, Huang RN, Wang C, et al: Epidemiology of Meningococcal Disease in Chengdu, 1950-2008. (In Chinese.). J Prev Med Inf. 2010, 26: 38-42.Google Scholar
- Hu XJ, Li XW, Ji YD, Xi W, Gao LH: Study on periodically prevalent features for epidemic cerebrospinal meningitis in China. J Chin Epidemiol. 1991, 12: 136-139.Google Scholar
- Jin YH, Wang B: The epidemiological trend of meningococcal disease in Hefei,1990–1999 (In Chinese.). Chinese J Dis Control Prevent. 2001, 5: 157-158.Google Scholar
- World Health Organization: Emerging and other communicable diseases Surveillance and control. of epidemic meningococcal disease. 1998, WHO, Geneva, 2Google Scholar
- Yang JF, Li JH, Li YX, et al: Prevention and control of epidemic meningococcal disease (In Chinese). Chinese Journal of Vaccination and Immunization. 2006, 12: 61-63.Google Scholar
- Liu DQ, Wang BB, Cheng X, et al: Epidemiological character and control measure for meningococcal disease in Anhui province during 2004–2005 (In Chinese.). Chinese Journal of Vaccination and Immunization. 2006, 12: 40-43.Google Scholar
- Ministry of Health of China: National monitoring and surveillance scheme on meningococcal disease. , , http://www.moh.gov.cn/open/uploadfile/200662715334853.doc (accessed 29 Jan 2008)
- Ruoff KL, Whiley RA, Beighton D, et al: Manual of clinical microbiology. 2003, ASM Press, Washington, DC, 405-421. 8Google Scholar
- Clinical and Laboratory Standards Institute/NCCLS: Performance standards for antimicrobial susceptibility testing; Sixteenth informational supplement. CLSI/NCCLS document M100-S17. Clinical and Laboratory Standards Institute Wayne Pennsylvania . 2008, 27: 1-163.Google Scholar
- Brooks R, Woods CW, Rosenstein NE: Increased case-fatality rate associated with outbreaks of Neisseria meningitidis infection, compared with sporadic meningococcal disease, in the United States, 1994-2002. Clin Infect Dis. 2006, 43: 49-54. 10.1086/504804.View ArticlePubMedGoogle Scholar
- Wu HM, Harcourt BH, Hatcher CP, et al: Emergence of ciprofloxacin-resistant Neisseria meningitidis in North America. N Engl J Med. 2009, 360: 886-892. 10.1056/NEJMoa0806414.View ArticlePubMedGoogle Scholar
- Aguilera JF, Perrocheau A, Meffre C, et al: Outbreak of serogroup W135 meningococcal disease after the Hajj pilgrimage, Europe, 2000. Emerg Infect Dis. 2002, 8: 761-767. 10.3201/eid0808.010422.View ArticlePubMedPubMed CentralGoogle Scholar
- Vogel U, Claus H, Frosch M: Rapid serogroup switching in Neisseria meningitidis. N Engl J Med. 2000, 342: 219-220. 10.1056/NEJM200001203420319.View ArticlePubMedGoogle Scholar
- Mayer LW, Reeves MW, Al-Hamdan N, et al: Outbreak of W135 meningococcal disease in 2000:not emergence of a new W135 strain but clonal expansion within the electophoretic type-37 complex. J Infect Dis. 2002, 185: 1596-1605. 10.1086/340414.View ArticlePubMedGoogle Scholar
- Zombre S, Hacen MM, Ouango G, et al: The outbreak of meningitis due to Neisseria meningitidis W135 in 2003 in Burkina Faso and the national response: main lessons learnt. Vaccine. 2007, 25: A69-A71.View ArticlePubMedGoogle Scholar
- Zhu JS, Wei L, Jun R, et al: Identification of a new Neisseria meningitidis serogroup C clone from Anhui province, China. Lancet. 2006, 367: 419-423. 10.1016/S0140-6736(06)68141-5.View ArticlePubMedGoogle Scholar
- Zhou H, Gao Y, Xu L, et al: Distribution of serogroups and sequence types in disease-associated and carrier strains of Neisseria meningitidis isolated in China between 2003 and 2008. Epidemiol Infect. 2011, 20: 1-8.Google Scholar
- Jensen ES, Schønheyder HC, Lind I, et al: Neisseria meningitidis phenotypic markers and septicaemia, disease progress and case-fatality rate of meningococcal disease: a 20-year population-based historical follow-up study in a Danish county. J Med Microbiol. 2003, 52: 173-179. 10.1099/jmm.0.04817-0.View ArticlePubMedGoogle Scholar
- Smith I, Caugant DA, Høiby EA, et al: High case-fatality rates of meningococcal disease in Western Norway caused by serogroup C strains belonging to both sequence type (ST)-32 and ST-11 complexes, 1985–2002. Epidemiol Infect. 2006, 134: 1195-1202. 10.1017/S0950268806006248.View ArticlePubMedPubMed CentralGoogle Scholar
- Rosenstein NE, Perkins BA, Stephens DS, et al: Meningococcal disease. N Engl J Med. 2001, 344: 1378-1388. 10.1056/NEJM200105033441807.View ArticlePubMedGoogle Scholar
- Castellanos-Ortega A, Delgado-Rodriguez M: Comparison of the performance of two general and three specific scoring systems for meningococcal septic shock in children. Crit Care Med. 2000, 28: 2967-2973. 10.1097/00003246-200008000-00047.View ArticlePubMedGoogle Scholar
- Thomson AP, Sills JA, Hart CA: Validation of the Glasgow Meningococcal Septicemia Prognostic Score: a 10-year retrospective survey. Crit Care Med. 1991, 19: 26-30. 10.1097/00003246-199101000-00010.View ArticlePubMedGoogle Scholar
- Barquet N, Domingo P, Cayla JA, et al: Prognostic factors in meningococcal disease. Development of a bedside predictive model and scoring system. Barcelona Meningococcal Disease Surveillance Group. JAMA. 1997, 278: 491-496. 10.1001/jama.1997.03550060067038.View ArticlePubMedGoogle Scholar
- van Deuren M, Brandtzaeg P, van der Meer JW: Update on meningococcal disease with emphasis on pathogenesis and clinical management. Clin Microbiol Rev. 2000, 13: 144-166. 10.1128/CMR.13.1.144-166.2000.View ArticlePubMedPubMed CentralGoogle Scholar
- Harrison LH, Pass MA, Mendelsohn AB, et al: Invasive meningococcal in adolescents and young adults. JAMA. 2001, 286: 694-699. 10.1001/jama.286.6.694.View ArticlePubMedGoogle Scholar
- The Meningococcal Disease Surveillance Group: Analysis of disease by serogroup and evaluation of chemoprophylaxis. J Infect Dis. 1976, 134: 201-204.View ArticleGoogle Scholar
- Peltola H, Jonsdottir K, Lystad A, et al: Meningococcal disease in Scandinavia. Br Med J (Clin Res Ed). 1982, 284: 1618-1621. 10.1136/bmj.284.6329.1618.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/12/205/prepub
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