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Figure 3 | BMC Infectious Diseases

Figure 3

From: Identification of an N-terminal 27 kDa fragment of Mycoplasma pneumoniae P116 protein as specific immunogen in M. pneumoniaeinfections

Figure 3

Immunogenicity and specificity of P116 (N-27) and P1 (C-40) proteins and their antibodies. A. Bar diagram showing Immune response in two different White NewZealand rabbits immunized with purified recombinant P116(N-27) and P1(C-40) proteins. Control rabbits were injected with complete and incomplete Freund's adjuvant in normal saline according to the immunization schedule. B. Immunoblot analysis of M. pneumoniae lysate showing reactivity of P1(179 kDa) with anti-P1 serum raised in rabbit-Lane1: M. pneumoniae lysate, Lane 2: Blank, Lane 3: Prestained standard protein marker. C. Immunoblot analysis of M. pneumoniae lysate showing reactivity of P116(116 kDa) with anti-P116 serum raised in rabbit-Lane 1: Prestained standard protein marker, Lane 2: Blank, Lane 3: M. pneumoniae lysate

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