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Birth cohort effect on latent tuberculosis infection prevalence, United States
© Winston and Navin; licensee BioMed Central Ltd. 2010
Received: 1 February 2010
Accepted: 13 July 2010
Published: 13 July 2010
Latent tuberculosis infection (LTBI) prevalence in the United States decreased approximately 60% in the three decades between the 1971-1972 and 1999-2000 National Health and Nutrition Examination Survey (NHANES) surveys. We examined the effects of birth cohort on LTBI prevalence over time.
Using weighted data analysis software to account for NHANES survey design, we calculated the difference in LTBI prevalence between 1971-1972 and 1999-2000 for birth cohorts corresponding to 5-year intervals (1912-1916, 1917-1921,1922-1926, 1927-1931, 1932-1936, 1937-1941, 1942-1946).
LTBI prevalence was significantly lower in 1999-2000 compared to 1971-1972 for cohorts born in 1926 or earlier (19% versus 5%), but not for cohorts born 1927-1946 (9% versus 7%). Adjustment for cohort restriction and foreign-birth did not qualitatively change the results.
Although older age groups have higher rates of TB infection than younger groups, nationally representative U.S. survey data suggest that observed LTBI prevalence in older people represents an underestimate of infection, because of the birth cohort effect and waning immunologic reactivity.
Two recent publications [1, 2] estimate latent tuberculosis infection (LTBI) prevalence in the United States using the 1971-1972 and 1999-2000 National Health and Nutrition Examination Survey (NHANES) data. These studies show that LTBI prevalence has decreased approximately 60% in the three decades between the two surveys. Older age was associated with higher LTBI prevalence in both NHANES time periods; however, neither study examined LTBI in the same birth cohorts over time. Therefore, we compared NHANES 1971-1972 data with NHANES 1999-2000 data to examine the specific effects of birth cohort on LTBI prevalence.
Cohorts corresponding to birth years were constructed in 5-year intervals (1912-1916, 1917-1921,1922-1926, 1927-1931, 1932-1936, 1937-1941, 1942-1946) based on available 1971-1972 and 1999-2000 NHANES data by age. Only people age 25-74 years old were offered tuberculin skin testing (TST) in 1971-1972; all individuals 1 year of age and older were tested in 1999-2000. The 1999-2000 data were available only with top-coded age values such that all persons above age 85 were listed as age 85. Thus, we could not report comparisons for people born before 1912, and the comparison for people who in 1971-1972 were age 55-59 years (1912-1916 birth cohort) includes in 1999-2000 all people age 83 or older (born in 1916 or earlier). We report data by birth cohort years and corresponding ages.
Our outcome was LTBI prevalence based on TST positivity, defined as an induration of 10 mm or greater in reaction to purified protein derivative S-1. The protocol for the tuberculosis component of the NHANES was reviewed and approved by the NHANES institutional review board. We analyzed data using SUDAAN 9.0 (RTI International, Research Triangle Park, NC) and SAS 9.1 (SAS Institute, Cary, NC) software to account for NHANES sample survey design and weighting. In brief, we used NHANES Medical Examination Center sample weights to estimate prevalence proportions while accounting for unequal selection and interview and physical examination probabilities. We adjusted for TST nonparticipation according to methods of Bennett et al. .
For statistical comparisons, we calculated the difference in prevalence estimates between 1971-1972 and 1999-2000 for each cohort. We used sample variances derived from weighted data to calculate 95% confidence intervals (CI) around prevalence estimates and around prevalence differences. Prevalence differences comparing 1999-2000 with 1971-1972 estimates were considered statistically significant if the 95% CI excluded 0.
Latent TB infection prevalence by birth cohort
In 1971-1972 NHANES data, 1012 adults were born between 1912-1946 out of 1492 adults age 25-74 years with a TST result. Out of 7386 persons with a TST result in the 1999-2000 NHANES, 1710 adults were born before 1947.
Latent tuberculosis infection prevalence by birth cohort and sample year
Age in years in 1971-1972
Age in years in 1999-2000
Latent tuberculosis infection prevalence 1971-1972
Latent tuberculosis infection prevalence 1999-2000
95% Confidence interval (CI) around difference
(no. = 129)
(no. = 245)
(no. = 123)
(no. = 293)
(no. = 130)
(no. = 321)
(no. = 110)
(no. = 270)
(no. = 179)
(no. = 217)
(no. = 191)
(no. = 214)
(no. = 150)
(no. = 150)
Selection bias analysis comparing ages available in 1971-1972 vs. 1999-2000
TOTAL of cohorts
TOTAL of cohorts
(no. = 1012)
(no. = 1710)
(no. = 1492)
(no. = 3012)
TOTAL population tested
TOTAL population tested
(no. = 1492)
(no. = 7386)
Assessment of prevalence bias
To assess potential bias due to cohort restriction, we compared LTBI prevalence of the overall NHANES populations tested to persons eligible for our analysis. Including older persons (born before 1912) in the 1971-1972 population yielded nominally higher LTBI estimates than our cohort eligible population, while including younger persons (born after 1946) in 1999-2000 resulted in slightly lower LTBI estimates than our cohort analysis (Table 1).
When comparing 1971-1972 with 1999-2000 NHANES data, we found a significant decline in LTBI prevalence among people born before 1926 but not for younger birth cohorts. Differences in LTBI prevalence in a birth cohort over time are a balance between new infections and waning of immunologic response to previous infections [3, 4]. Our data suggest that for older birth cohorts, the balance is in favor of waning response to previous infections. For younger birth cohorts, the acquisition of new infections may tip the balance in the other direction.
Loss of immunologic reactivity to Mycobacterium tuberculosis in humans can be demonstrated by "two-step testing" with purified protein derivative (PPD), such as during repeated screening of healthcare workers. People who test negative in response to the first PPD test and subsequently test positive after a second test within a short period of time (~ 2 weeks) are thought to represent immunologic boosting of a previous infection . Some studies have reported that PPD boosting is associated with age. Among hospital workers in Saudi Arabia, immunologic boosting was more common among workers over the age of 45 than younger workers . Similarly, boosting was more than three times as common in employees 45 years and older than younger employees in a study of 10 hospitals throughout the United States . In contrast, a study in young (mean 21.4 years) healthcare workers in Montreal did not find an association between boosting and age . Perhaps waning reactivity to PPD is not observable before a certain age, such as 45 years.
A potential limitation of our analysis is that LTBI prevalence estimates were based on reaction to PPD S-1, which could include false-positive results based on reactivity to nontuberculous mycobacteria (NTM) or bacillus Calmette-Guérin (BCG) vaccination [8, 9], An alternate definition of LTBI incorporating results for PPD-Battey in addition to PPD S-1 to assess NTM cross-reactivity yielded no significant differences in prevalence estimates compared with LTBI based on PPD S-1 alone . Furthermore, the 1999-2000 NHANES reported a higher prevalence of NTM sensitization compared with the 1971-1972 NHANES, and this difference was significant among foreign-born persons . Increases over time in reactivity to NTM would increase LTBI prevalence estimates, rather than contribute to decreases in LTBI that we report. In addition, the waning effect of BCG vaccination is not likely to bias our findings. Prevalence estimates considering foreign-born individuals with a BCG scar as not having LTBI did not differ from estimates ignoring BCG scars ; moreover, the NHANES foreign-born population was larger in 1999-2000 than 1971-1972, thus, potential misclassification due to BCG would result in higher, rather than lower, LTBI estimates in 1999-2000. Nonetheless, future research on LTBI will benefit from newer diagnostic technologies with higher specificity for M. tuberculosis, such as interferon gamma release assays. We did not analyze data separately for persons who had ever been prescribed treatment for TB or LTBI since this group represented only 1.3% of the total population in 1999-2000 NHANES [1, 2].
The reduced LTBI prevalence among earlier birth cohorts that we observed could be influenced by selective mortality, to the degree to which people with LTBI progress to TB disease and die from TB or comorbid conditions. Since LTBI is more prevalent among vulnerable populations , people with high rates of LTBI in earlier birth cohorts may experience higher mortality, resulting in lower LTBI rates among survivors. We cannot directly evaluate the influence of self-cure of TB, waning immunity, or selective mortality, since we compared birth cohorts across NHANES sample years, rather than longitudinal follow-up of the same individuals over time. Another limitation was small sample size in some cells, which restricted our ability to stratify analyses. Finally, data were not available for a detailed analysis of people born before 1912 or after 1946. However, comparison of LTBI prevalence -- a relatively rare outcome -- is strengthened by our use of large, nationally representative samples with consistent TST protocols. NHANES household sampling mitigates potential bias, in that the most vulnerable populations for LTBI (homeless or incarcerated people, and residents of long-term care facilities) are excluded. Importantly, our findings did not appear to be driven by greater inclusion of foreign-born people in 1999-2000 NHANES, as results were similar when restricted to the U.S.-born.
Our study is the first that we are aware of to describe the change in LTBI prevalence associated with age in relationship to the effects of birth cohort, as previously described with respect to TB disease . Although previous studies have shown that older age groups have higher rates of TB infection than younger groups, our nationally representative data suggest that observed LTBI prevalence in older people represents an underestimate of infection, because of the birth cohort effect and waning immunologic reactivity.
We acknowledge Jeanne M. Courval for developing TST non-participation weights, for which methods have been published . Funding provided solely by the U.S. Centers for Disease Control and Prevention. The findings and conclusions in this publication are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
- Bennett DE, Courval JM, Onorato I, Agerton T, Gibson JD, Lambert L, McQuillan GM, Lewis B, Navin TR, Castro KG: Prevalence of tuberculosis infection in the United States population: The National Health and Nutrition Examination Survey, 1999-2000. Am J Resp Crit Care Med. 2008, 177: 348-355. 10.1164/rccm.200701-057OC.View ArticlePubMedGoogle Scholar
- Khan K, Wang J, Hu W, Bierman A, Li Y, Gardam M: Tuberculosis infection in the United States: national trends over three decades. Am J Resp Crit Care Med. 2008, 177: 455-460. 10.1164/rccm.200706-950OC.View ArticlePubMedGoogle Scholar
- Fitzgerald D, Haas D: Myobacterium tuberculosis. Principles and Practice of Infectious Diseases. Edited by: Mandell GL, Bennett JE, and R Dolin. 2005, Philadelphia, PA, Churchill Livingston, 2852-86. 6Google Scholar
- Zevallos M, Justman JE: Tuberculosis in the elderly. Clin Geriatr Med. 2003, 19: 121-123. 10.1016/S0749-0690(02)00057-5.View ArticlePubMedGoogle Scholar
- Al Mazrou AM: Booster effect of two-step tuberculin skin testing among hospital employees from areas with a high prevalence of tuberculosis. Infect Control Hosp Epidemiol. 2004, 25: 1117-1120. 10.1086/502355.View ArticlePubMedGoogle Scholar
- Snider DE, Cauthen GM: Tuberculin skin testing of hospital employees: infection, "boosting," and two-step testing. Am J Infect Control. 1984, 12: 305-311. 10.1016/0196-6553(84)90001-4.View ArticlePubMedGoogle Scholar
- Menzies R, Vissandjee B, Rocher I, St. Germain Y: The booster effect in two-step tuberculin testing among young adults in Montreal. Ann Intern Med. 1994, 120: 190-198.View ArticlePubMedGoogle Scholar
- Smith DT: Diagnostic and prognostic significance of the quantitative tuberculin skin tests. Ann Intern Med. 1967, 67: 919-946.View ArticlePubMedGoogle Scholar
- CDC: Targeted tuberculin testing and treatment of latent tuberculosis infection. MMWR. 2000, 49: 1-54. RR06Google Scholar
- Khan K, Wang J, Marras TK: Nontuberculous mycobacterial sensitization in the United States: national trends over three decades. Am J Respir Crit Care Med. 2007, 176: 306-313. 10.1164/rccm.200702-201OC.View ArticlePubMedGoogle Scholar
- CDC: Guidelines for using the QuantiFERON-TB Gold test for detecting Mycobacterium tuberculosis infection, United States. MMWR. 2005, 54: 49-55.Google Scholar
- Frost WH: The age selection of mortality from tuberculosis in successive decades. Am J Hyg. 1939, 30: 91-96.Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/10/206/prepub
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