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Table 3 PCR Cycling Conditions: HLA DRB gene

From: Non-detection of Chlamydia species in carotid atheroma using generic primers by nested PCR in a population with a high prevalence of Chlamydia pneumoniae antibody

Cycle Conditions First Round
PrePCRdenaturation 96°C – 5 min
Denaturation 96°C – 1 min
Annealing 55 °C – 1 min
Extension 72°C – 1 min
Number of Cycles 40