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Table 2 PCR Cycling Conditions: Chlamydia genus specific

From: Non-detection of Chlamydia species in carotid atheroma using generic primers by nested PCR in a population with a high prevalence of Chlamydia pneumoniae antibody

Cycle Conditions First Round Second Round
PrePCRdenaturation 94°C – 3 min 94°C – 2 min
Denaturation 94°C – 10 s 94°C – 10 s
Annealing 45°C – 10 s 45°C – 10 s
Extension 72°C – 30 s 72°C – 30 s
Number of Cycles 35 35